HCN2-IN-4
HCN2-IN-4 is an orally active HCN2 ion channel inhibitor, with an IC50 of 3 nM. HCN2-IN-4 inhibits the activity of HCN2 ion channels. HCN2-IN-4 can be used in the research of pain, tinnitus and central nervous system diseases.
For research use only. We do not sell to patients.
- CAS No.: 3080809-13-5
- Formula: C21H18FN5O4S
- Molecular Weight:455.46
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
HCN2-IN-4 (Compound 49) potently and selectively inhibits human HCN2 channels, with an IC50 of 3 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 3080809-13-5
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Molecular Weight 455.46
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Formula C21H18FN5O4S
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SMILES
O=C(C1=CC=NS1)NC2=NN(C[C@@H](C)OC)C3=NC=C(C4=CC=C(OCO5)C5=C4F)C=C23
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Two-electrode voltage clamp in Xenopus oocytes
Two-electrode voltage clamp measures whole-oocyte membrane current from Xenopus oocytes expressing exogenous ion channels, receptors, or transporters; one intracellular microelectrode senses membrane voltage, and the second injects current so the amplifier can hold the membrane at command voltages while recording the compensating current as the functional readout. The method is suited to Xenopus oocytes because their large size supports microinjection and intracellular electrode impalement, but the large membrane area can limit voltage-clamp speed and accuracy, especially for large or fast currents.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HCN2-IN-4 Related Classifications
HY-186061 Related Classifications
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