Heparinase II

Product Information
Calcium ions inhibit the activity of heparinase II. Heparinase II cleaves sulfated polysaccharide chains containing 1-4 linkages between hexosamine and uronic acid residues (including iduronic acid and glucuronic acid residues) by an elimination mechanism. The reaction generates oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids, which can be detected by UV spectroscopy at 232 nm. The enzyme also cleaves the antithrombin III binding pentasaccharide domain in the heparin molecule.

Dissolution
Dissolved in water at 1 mg/mL, fresh preparation is recommended. Or dissolve according to the specific experimental reference.

Reaction Conditions
1. pH stability range 5.0-9.0, optimum pH 7.0-7.5
2. Temperature stability range 20-37°C, optimum temperature 30°C
Note: When enzymatically cleaving the substrate, the incubation time and enzyme concentration need to be determined based on actual experience.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.