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Cat. No.: HY-152103

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CB2-H

CB2-H is a dual-channel fluorescent probe for the simultaneous detection of HOCl and ONOO^-. CB2-H enables the concurrent detection of HOCl and ONOO^- at two independent channels without spectral cross-interference and can be applied for dual-channel fluorescence imaging of endogenously produced HOCl and ONOO^- in living cells and zebrafish under different stimulants.

For research use only. We do not sell to patients.

Formula: C₂₉H₂₃Br₂N₃O₅
Molecular Weight:653.32

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

CB2-H Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

ONOO^- can selectively oxidize the hydrazide group of CB2-H to afford the parent dye CB2 (Abs_max/Em_max = 631/669 nm). In the case of HOCl, it undergoes an electrophilic attack on the benzopyran moiety of CB2-H to give a chlorinated product CB2-H-Cl, which inhibits the PET process within the probe and thus affords a turn-on fluorescence response at the coumarin channel (Abs_max/Em_max = 407/468 nm)^[1].
CB2-H (5 μM; 30 min) is capable of concurrently monitoring endogenously produced HOCl and ONOO^- in living cells and zebrafish under different stimulants^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 653.32
Formula C₂₉H₂₃Br₂N₃O₅
SMILES

CCN(C1=CC=C2C(OC(C3=CC4=C(C(Br)=C(C(Br)=C4)O)OC3=O)=CC25C6=C(C(N5N)=O)C=CC=C6)=C1)CC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Huang W, et al. Rational Design of a Dual-Channel Fluorescent Probe for the Simultaneous Imaging of Hypochlorous Acid and Peroxynitrite in Living Organisms. Anal Chem. 2022 Dec 20;94(50):17485-17493. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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