Lepidiline C
Based on 1 Customer Validation
Lepidiline C shows cytotoxic activity against HL-60 cells with an IC50 of 27.7 μM.
For research use only. We do not sell to patients.
- Purity : 98.60%
- CAS No.: 2750933-59-4
- Formula: C20H23ClN2O
- Molecular Weight:342.86
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | IC50 |
27.7 μM
Compound: 1c
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Cytotoxicity against human HL-60 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
Cytotoxicity against human HL-60 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
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[PMID: 34808062] |
| HUVEC | IC50 |
>100 μM
Compound: 1c
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Cytotoxicity against HUVEC cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
Cytotoxicity against HUVEC cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
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[PMID: 34808062] |
| MCF7 | IC50 |
75 μM
Compound: 1c
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Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay
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[PMID: 34808062] |
Chemical Information
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CAS No. 2750933-59-4
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Appearance Solid
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Molecular Weight 342.86
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Formula C20H23ClN2O
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Color White to off-white
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SMILES
CC1=C(N(C=[N+]1CC2=CC=CC=C2)CC3=CC=CC(OC)=C3)C.[Cl-]
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (291.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9166 mL | 14.5832 mL | 29.1664 mL | 72.9161 mL |
| 5 mM | 0.5833 mL | 2.9166 mL | 5.8333 mL | 14.5832 mL | |
| 10 mM | 0.2917 mL | 1.4583 mL | 2.9166 mL | 7.2916 mL | |
| 15 mM | 0.1944 mL | 0.9722 mL | 1.9444 mL | 4.8611 mL | |
| 20 mM | 0.1458 mL | 0.7292 mL | 1.4583 mL | 3.6458 mL | |
| 25 mM | 0.1167 mL | 0.5833 mL | 1.1667 mL | 2.9166 mL | |
| 30 mM | 0.0972 mL | 0.4861 mL | 0.9722 mL | 2.4305 mL | |
| 40 mM | 0.0729 mL | 0.3646 mL | 0.7292 mL | 1.8229 mL | |
| 50 mM | 0.0583 mL | 0.2917 mL | 0.5833 mL | 1.4583 mL | |
| 60 mM | 0.0486 mL | 0.2431 mL | 0.4861 mL | 1.2153 mL | |
| 80 mM | 0.0365 mL | 0.1823 mL | 0.3646 mL | 0.9115 mL | |
| 100 mM | 0.0292 mL | 0.1458 mL | 0.2917 mL | 0.7292 mL |
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