Isoallolithocholic acid
Based on 3 publication(s) in Google Scholar
Isoallolithocholic acid (3β-Hydroxy-5α-cholanic acid), a derivative of Lithocholic acid (HY-10219), is a T cell regulator. Isoallolithocholic acid enhances regulatory T cells (Tregs) differentiation.
For research use only. We do not sell to patients.
- Purity: 99.84%
- CAS No.: 2276-93-9
- Formula: C24H40O3
- Molecular Weight:376.57
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Isoallolithocholic acid
More
Biological Activity
Isoallolithocholic acid (3β-Hydroxy-5α-cholanic acid) (20 μM) reduces Th17 cell differentiation by ~50% without affecting RORγt expression[1].
Isoallolithocholic acid does not affect T cell differentiation into Th1 or Th2 cells, the modulatory effect of Isoallolithocholic acid on Tregs is cell-type specific[1].
Isoallolithocholic acid-dependent enhanced expression of FoxP3 requires mitoROS[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Segmented filamentous bacteria (SFB)-colonized Jax-B6 mice[1]
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Dosage:0.03% (w/w)
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Administration:In diet, 7 days
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Result:Was insufficient to enhance Treg percentages both at steady state and following anti-CD3 treatment alone. Significantly enhanced the Treg population in mice treated with anti-CD3 compared to control diet in combination with 0.3% (w/w) 3-oxolithocholic acid (3-oxoLCA). Reduced the number of CD45.1+ T effector cells.
Chemical Information
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CAS No. 2276-93-9
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Appearance Solid
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Molecular Weight 376.57
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Formula C24H40O3
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Color White to off-white
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SMILES
C[C@H](CCC(O)=O)[C@H]1CC[C@@]2([H])[C@]3([H])CC[C@@]4([H])C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
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Synonyms
3β-Hydroxy-5α-cholanic acid
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 83.33 mg/mL (221.29 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.52 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6555 mL | 13.2777 mL | 26.5555 mL | 66.3887 mL |
| 5 mM | 0.5311 mL | 2.6555 mL | 5.3111 mL | 13.2777 mL | |
| 10 mM | 0.2656 mL | 1.3278 mL | 2.6555 mL | 6.6389 mL | |
| 15 mM | 0.1770 mL | 0.8852 mL | 1.7704 mL | 4.4259 mL | |
| 20 mM | 0.1328 mL | 0.6639 mL | 1.3278 mL | 3.3194 mL | |
| 25 mM | 0.1062 mL | 0.5311 mL | 1.0622 mL | 2.6555 mL | |
| 30 mM | 0.0885 mL | 0.4426 mL | 0.8852 mL | 2.2130 mL | |
| 40 mM | 0.0664 mL | 0.3319 mL | 0.6639 mL | 1.6597 mL | |
| 50 mM | 0.0531 mL | 0.2656 mL | 0.5311 mL | 1.3278 mL | |
| 60 mM | 0.0443 mL | 0.2213 mL | 0.4426 mL | 1.1065 mL | |
| 80 mM | 0.0332 mL | 0.1660 mL | 0.3319 mL | 0.8299 mL | |
| 100 mM | 0.0266 mL | 0.1328 mL | 0.2656 mL | 0.6639 mL |
Isoallolithocholic acid Related Classifications
HY-B0172A Related Classifications
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