Isolithocholic acid
Based on 1 publication(s) in Google Scholar
Isolithocholic acid (β-Lithocholic acid) is an isomer of Lithocholic acid. Isolithocholic acid, a bile acid, is formed by microbial metabolism of Lithocholic acid or Lithocholic acid 3α-sulfate.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 1534-35-6
- Formula: C24H40O3
- Molecular Weight:376.57
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Isolithocholic acid
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
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Microbial Metabolite |
Isolithocholic acid at 0.01 % does not inhibit spore germination and outgrowth of CF5 and M120, but it significantly inhibits at the higher concentration (0.1 %). Isolithocholic acid (0.00003 %) prevents growth of CD196, M68, CF5, 630, and BI9 and significant decreases strains CF5, BI9, M120, and 630 in toxin activity. Isolithocholic acid (0.0003 %) makes that all strains displays a significant decrease in toxin activity, except for R20291 and M120[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1534-35-6
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Appearance Solid
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Molecular Weight 376.57
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Formula C24H40O3
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Color White to off-white
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SMILES
C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCC(O)=O)([H])[C@@]3([H])[C@@](CC1)([H])[C@@]4([C@](C[C@H](CC4)O)([H])CC3)C
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Synonyms
3β-Hydroxy-5β-cholanic acid; 3-Epilithocholic acid; β-Lithocholic acid
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Food Funct
Lactobacillus_rhamnosus GG reverses zearalenone-induced pathological macrophage polarization to restore placental angiogenesis and efficiency. [Abstract]2026 Jun 22;17(12):5425-5438. PMID: 42300468
Solvent & Solubility
DMSO : 25 mg/mL (66.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Batta AK, et al. Transformation of bile acids into iso-bile acids by Clostridium perfringens: possible transport of 3 beta-hydrogen via the coenzyme. Hepatology. 1985;5(6):1126-1131. [Content Brief]
[2]. Borriello SP, et al. The metabolism of lithocholic acid and lithocholic acid-3-alpha-sulfate by human fecal bacteria. Lipids. 1982;17(7):477-482. [Content Brief]
[3]. Thanissery R, et al. Inhibition of spore germination, growth, and toxin activity of clinically relevant C. difficile strains by gut microbiota derived secondary bile acids. Anaerobe. 2017;45:86-100. [Content Brief]
[4]. Lin H, et al. Alterations of Bile Acids and Gut Microbiota in Obesity Induced by High Fat Diet in Rat Model. J Agric Food Chem. 2019;67(13):3624-3632. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.6555 mL | 13.2777 mL | 26.5555 mL | 66.3887 mL |
| 5 mM | 0.5311 mL | 2.6555 mL | 5.3111 mL | 13.2777 mL | |
| 10 mM | 0.2656 mL | 1.3278 mL | 2.6555 mL | 6.6389 mL | |
| 15 mM | 0.1770 mL | 0.8852 mL | 1.7704 mL | 4.4259 mL | |
| 20 mM | 0.1328 mL | 0.6639 mL | 1.3278 mL | 3.3194 mL | |
| 25 mM | 0.1062 mL | 0.5311 mL | 1.0622 mL | 2.6555 mL | |
| 30 mM | 0.0885 mL | 0.4426 mL | 0.8852 mL | 2.2130 mL | |
| 40 mM | 0.0664 mL | 0.3319 mL | 0.6639 mL | 1.6597 mL | |
| 50 mM | 0.0531 mL | 0.2656 mL | 0.5311 mL | 1.3278 mL | |
| 60 mM | 0.0443 mL | 0.2213 mL | 0.4426 mL | 1.1065 mL |
Isolithocholic acid Related Classifications
HY-B0172B Related Classifications
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