Cat. No.: HY-146506

isoCA-4

isoCA-4, a Combretastatin A4 derivative, is a tubulin polymerization inhibitor. isoCA-4 has anti-proliferative activities.

For research use only. We do not sell to patients.

CAS No.: 1067880-31-2
Formula: C₁₈H₂₀O₅
Molecular Weight:316.35

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Data Sheet (266 KB)

isoCA-4 Chemical Structure

CAS No.: 1067880-31-2

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Biological Activity
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Chemical Information
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Biological Activity

Description

isoCA-4, a Combretastatin A4 derivative, is a tubulin polymerization inhibitor. isoCA-4 has anti-proliferative activities^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	26 nM Compound: isoCA-4	Antiproliferative activity against human A2780 cells after 48 hrs by MTT assay Antiproliferative activity against human A2780 cells after 48 hrs by MTT assay	[PMID: 28792756]
A2780	IC₅₀	3.01 nM Compound: isoCA-4	Cytotoxicity against human A2780 cells assessed as reduction in cell growth measured after 72 hrs by MTS assay Cytotoxicity against human A2780 cells assessed as reduction in cell growth measured after 72 hrs by MTS assay	[PMID: 30818177]
A549	IC₅₀	8.2 nM Compound: isoCA-4	Cytotoxicity against human A549 cells assessed as decrease in cell growth after 72 hrs by resazurin dye-based fluorimetric method Cytotoxicity against human A549 cells assessed as decrease in cell growth after 72 hrs by resazurin dye-based fluorimetric method	[PMID: 28166995]
A549	IC₅₀	3.5 nM Compound: isoCA-4	Antiproliferative activity against human A549 cells after 48 hrs by MTT assay Antiproliferative activity against human A549 cells after 48 hrs by MTT assay	[PMID: 28792756]
A549	IC₅₀	20 nM Compound: isoCA-4	Antiproliferative activity against human A549 cells after 72 hrs by resazurin-based fluorescence assay Antiproliferative activity against human A549 cells after 72 hrs by resazurin-based fluorescence assay	[PMID: 29202409]
A549	GI₅₀	10 nM Compound: 3a; isoCA-4	Antiproliferative activity against human A549 cells after 72 hrs by MTS assay Antiproliferative activity against human A549 cells after 72 hrs by MTS assay	[PMID: 30004697]
A549	IC₅₀	8 nM Compound: iso-CA-4	Antiproliferative activity against human A549 cells after 72 hrs by MTS assay Antiproliferative activity against human A549 cells after 72 hrs by MTS assay	[PMID: 30525602]
A549	IC₅₀	10 nM Compound: 3; isoCA4	Antiproliferative activity against human A549 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay Antiproliferative activity against human A549 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay	[PMID: 33038796]
A549	IC₅₀	52.7 nM Compound: 3; isoCA-4	Cytotoxicity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 34171660]
A549	IC₅₀	12 nM Compound: isoCA4	Antiproliferative activity against human A549 cells after 72 hrs by CellTiter Glo assay Antiproliferative activity against human A549 cells after 72 hrs by CellTiter Glo assay	[PMID: 35797900]
A549/CDDP	IC₅₀	69.1 nM Compound: isoCA-4	Antiproliferative activity against human A549/CDDP cells after 48 hrs by MTT assay Antiproliferative activity against human A549/CDDP cells after 48 hrs by MTT assay	[PMID: 28792756]
BXPC-3	GI₅₀	20 nM Compound: 3a; isoCA-4	Antiproliferative activity against human BxPC3 cells after 72 hrs by MTS assay Antiproliferative activity against human BxPC3 cells after 72 hrs by MTS assay	[PMID: 30004697]
H22	IC₅₀	0.008 μM Compound: isoCA-4; 2	Antiproliferative activity against mouse H22 cells assessed as inhibition of cell growth by MTT assay Antiproliferative activity against mouse H22 cells assessed as inhibition of cell growth by MTT assay	[PMID: 32339853]
HCT-116	IC₅₀	2.5 nM Compound: isoCA-4	Cytotoxicity against human HCT116 cells after 72 hrs Cytotoxicity against human HCT116 cells after 72 hrs	[PMID: 19530698]
HCT-116	IC₅₀	2 nM Compound: isoCA-4	Cytotoxicity against human HCT116 cells after 72 hrs Cytotoxicity against human HCT116 cells after 72 hrs	[PMID: 20627378]
HCT-116	IC₅₀	2 x 10^-9 M Compound: isoCA-4	Cytotoxicity against human HCT116 cells after 72 hrs Cytotoxicity against human HCT116 cells after 72 hrs	[PMID: 20627378]
HCT-116	GI₅₀	3 nM Compound: isoCA-4	Cytotoxicity against human HCT116 cells after 72 hrs using resazurin dye by microtiter plate fluorimeter analysis Cytotoxicity against human HCT116 cells after 72 hrs using resazurin dye by microtiter plate fluorimeter analysis	[PMID: 22449653]
HCT-116	GI₅₀	3 nM Compound: 2a, isoCA-4	Growth inhibition of human HCT116 cells after 72 hrs by Victor microtiter plate fluorimeter analysis Growth inhibition of human HCT116 cells after 72 hrs by Victor microtiter plate fluorimeter analysis	[PMID: 23353744]
HCT-116	GI₅₀	2 nM Compound: isoCA-4	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by resazurin dye-based fluorimetric analysis Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by resazurin dye-based fluorimetric analysis	[PMID: 24681982]
HCT-116	GI₅₀	2.2 nM Compound: isoCA-4	Cytotoxicity against human HCT116 cells after 72 hrs by CellTitre-Blue assay Cytotoxicity against human HCT116 cells after 72 hrs by CellTitre-Blue assay	[PMID: 25528337]
HCT-116	IC₅₀	2 nM Compound: isoCA-4	Cytotoxicity against human HCT116 cells assessed as decrease in cell growth after 72 hrs by resazurin dye-based fluorimetric method Cytotoxicity against human HCT116 cells assessed as decrease in cell growth after 72 hrs by resazurin dye-based fluorimetric method	[PMID: 28166995]
HCT-116	IC₅₀	24 nM Compound: isoCA-4	Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 28792756]
HCT-116	IC₅₀	2 nM Compound: isoCA-4	Antiproliferative activity against human HCT116 cells after 72 hrs by resazurin-based fluorescence assay Antiproliferative activity against human HCT116 cells after 72 hrs by resazurin-based fluorescence assay	[PMID: 29202409]
HCT-116	GI₅₀	2 nM Compound: 3a; isoCA-4	Antiproliferative activity against human HCT116 cells after 72 hrs by MTS assay Antiproliferative activity against human HCT116 cells after 72 hrs by MTS assay	[PMID: 30004697]
HCT-116	IC₅₀	2 nM Compound: iso-CA-4	Antiproliferative activity against human HCT116 cells after 72 hrs by MTS assay Antiproliferative activity against human HCT116 cells after 72 hrs by MTS assay	[PMID: 30525602]
HCT-116	IC₅₀	1.8 nM Compound: isoCA-4	Cytotoxicity against human HCT116 cells assessed as reduction in cell growth measured after 72 hrs by MTS assay Cytotoxicity against human HCT116 cells assessed as reduction in cell growth measured after 72 hrs by MTS assay	[PMID: 30818177]
HCT-116	IC₅₀	2.4 nM Compound: 3; isoCA4	Antiproliferative activity against human HCT116 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay Antiproliferative activity against human HCT116 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay	[PMID: 33038796]
HCT-116	IC₅₀	0.64 nM Compound: 3; isoCA-4	Cytotoxicity against human HCT-116 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HCT-116 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 34171660]
HCT-116	GI₅₀	2.2 nM Compound: isoCA4	Antiproliferative activity against human HCT-116 cells after 72 hrs by CellTiter Glo assay Antiproliferative activity against human HCT-116 cells after 72 hrs by CellTiter Glo assay	[PMID: 35797900]
HeLa	IC₅₀	6 nM Compound: isoCA-4	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 28792756]
HeLa	IC₅₀	0.085 μM Compound: isoCA-4	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by SRB assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by SRB assay	[PMID: 28803799]
HepG2	IC₅₀	18.3 nM Compound: isoCA-4	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 28792756]
HepG2	IC₅₀	0.01 μM Compound: isoCA-4; 2	Antiproliferative against human HepG2 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Antiproliferative against human HepG2 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 32339853]
HT-1080	IC₅₀	0.91 nM Compound: 3; isoCA-4	Cytotoxicity against human HT-1080 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HT-1080 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 34171660]
HT-29	GI₅₀	276 nM Compound: 3a; isoCA-4	Antiproliferative activity against human HT-29 cells after 72 hrs by MTS assay Antiproliferative activity against human HT-29 cells after 72 hrs by MTS assay	[PMID: 30004697]
HT-29	IC₅₀	3.8 nM Compound: 3a; isoCA-4	Antiproliferative activity against human HT-29 cells in presence of TSA after 72 hrs by MTS assay relative to control Antiproliferative activity against human HT-29 cells in presence of TSA after 72 hrs by MTS assay relative to control	[PMID: 30004697]
HT-29	IC₅₀	265 nM Compound: iso-CA-4	Antiproliferative activity against human HT-29 cells after 72 hrs by MTS assay Antiproliferative activity against human HT-29 cells after 72 hrs by MTS assay	[PMID: 30525602]
HT-29	IC₅₀	275 nM Compound: 3; isoCA4	Antiproliferative activity against human HT-29 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay Antiproliferative activity against human HT-29 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay	[PMID: 33038796]
HT-29	IC₅₀	>100 nM Compound: 3; isoCA-4	Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 34171660]
HT-29	IC₅₀	275 nM Compound: isoCA4	Antiproliferative activity against human HT-29 cells after 72 hrs by CellTiter Glo assay Antiproliferative activity against human HT-29 cells after 72 hrs by CellTiter Glo assay	[PMID: 35797900]
HUVEC	IC₅₀	2 nM Compound: iso-CA-4	Antiproliferative activity against HUVEC after 72 hrs by MTS assay Antiproliferative activity against HUVEC after 72 hrs by MTS assay	[PMID: 30525602]
JIMT-1	IC₅₀	3.08 nM Compound: isoCA-4	Cytotoxicity against human JIMT1 cells assessed as reduction in cell growth measured after 72 hrs by MTS assay Cytotoxicity against human JIMT1 cells assessed as reduction in cell growth measured after 72 hrs by MTS assay	[PMID: 30818177]
K562	IC₅₀	5 nM Compound: isoCA-4	Cytotoxicity against human K562 cells after 72 hrs Cytotoxicity against human K562 cells after 72 hrs	[PMID: 19530698]
K562	GI₅₀	5 nM Compound: isoCA-4	Cytotoxicity against human K562 cells after 72 hrs using resazurin dye by microtiter plate fluorimeter analysis Cytotoxicity against human K562 cells after 72 hrs using resazurin dye by microtiter plate fluorimeter analysis	[PMID: 22449653]
K562	GI₅₀	5 nM Compound: 2a, isoCA-4	Growth inhibition of human K562 cells after 72 hrs by Victor microtiter plate fluorimeter analysis Growth inhibition of human K562 cells after 72 hrs by Victor microtiter plate fluorimeter analysis	[PMID: 23353744]
K562	GI₅₀	5.2 nM Compound: isoCA-4	Cytotoxicity against human K562 cells after 72 hrs by CellTitre-Blue assay Cytotoxicity against human K562 cells after 72 hrs by CellTitre-Blue assay	[PMID: 25528337]
K562	IC₅₀	0.8 nM Compound: isoCA-4	Cytotoxicity against human K562 cells assessed as decrease in cell growth after 72 hrs by resazurin dye-based fluorimetric method Cytotoxicity against human K562 cells assessed as decrease in cell growth after 72 hrs by resazurin dye-based fluorimetric method	[PMID: 28166995]
K562	IC₅₀	5 nM Compound: isoCA-4	Antiproliferative activity against human K562 cells after 72 hrs by resazurin-based fluorescence assay Antiproliferative activity against human K562 cells after 72 hrs by resazurin-based fluorescence assay	[PMID: 29202409]
K562	GI₅₀	5.2 nM Compound: 3a; isoCA-4	Antiproliferative activity against human K562 cells after 72 hrs by MTS assay Antiproliferative activity against human K562 cells after 72 hrs by MTS assay	[PMID: 30004697]
K562	IC₅₀	2 nM Compound: iso-CA-4	Antiproliferative activity against human K562 cells after 72 hrs by MTS assay Antiproliferative activity against human K562 cells after 72 hrs by MTS assay	[PMID: 30525602]
K562	IC₅₀	0.8 nM Compound: isoCA-4	Cytotoxicity against human K562 cells assessed as reduction in cell growth measured after 72 hrs by MTS assay Cytotoxicity against human K562 cells assessed as reduction in cell growth measured after 72 hrs by MTS assay	[PMID: 30818177]
K562	IC₅₀	5.2 nM Compound: 3; isoCA4	Antiproliferative activity against human K562 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay Antiproliferative activity against human K562 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay	[PMID: 33038796]
K562	IC₅₀	4.26 nM Compound: 3; isoCA-4	Cytotoxicity against human K562 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human K562 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 34171660]
K562	IC₅₀	3 nM Compound: isoCA4	Antiproliferative activity against imatinib-resistant human K562 cells over expressing MDR1 after 72 hrs by CellTiter Glo assay Antiproliferative activity against imatinib-resistant human K562 cells over expressing MDR1 after 72 hrs by CellTiter Glo assay	[PMID: 35797900]
K562	IC₅₀	5.2 nM Compound: isoCA4	Antiproliferative activity against human K562 cells after 72 hrs by CellTiter Glo assay Antiproliferative activity against human K562 cells after 72 hrs by CellTiter Glo assay	[PMID: 35797900]
K-562R	IC₅₀	1.1 nM Compound: isoCA-4	Cytotoxicity against human K562R cells assessed as decrease in cell growth after 72 hrs by resazurin dye-based fluorimetric method Cytotoxicity against human K562R cells assessed as decrease in cell growth after 72 hrs by resazurin dye-based fluorimetric method	[PMID: 28166995]
K-562R	IC₅₀	2.2 nM Compound: isoCA-4	Antiproliferative activity against human K562R cells after 72 hrs by resazurin-based fluorescence assay Antiproliferative activity against human K562R cells after 72 hrs by resazurin-based fluorescence assay	[PMID: 29202409]
K-562R	GI₅₀	2.2 nM Compound: 3a; isoCA-4	Antiproliferative activity against human K562R cells after 72 hrs by MTS assay Antiproliferative activity against human K562R cells after 72 hrs by MTS assay	[PMID: 30004697]
K-562R	IC₅₀	3 nM Compound: iso-CA-4	Antiproliferative activity against human K562R cells after 72 hrs by MTS assay Antiproliferative activity against human K562R cells after 72 hrs by MTS assay	[PMID: 30525602]
K-562R	IC₅₀	1.1 nM Compound: isoCA-4	Cytotoxicity against human K562R cells assessed as reduction in cell growth measured after 72 hrs by MTS assay Cytotoxicity against human K562R cells assessed as reduction in cell growth measured after 72 hrs by MTS assay	[PMID: 30818177]
K-562R	IC₅₀	3 nM Compound: 3; isoCA4	Antiproliferative activity against human K562R cells assessed as cell viability after 24 hrs by Celltiter-Glo assay Antiproliferative activity against human K562R cells assessed as cell viability after 24 hrs by Celltiter-Glo assay	[PMID: 33038796]
K-562R	IC₅₀	5.13 nM Compound: 3; isoCA-4	Cytotoxicity against human K-562R cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human K-562R cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 34171660]
LoVo	IC₅₀	3.7 nM Compound: isoCA-4	Antiproliferative activity against human LoVo cells after 48 hrs by MTT assay Antiproliferative activity against human LoVo cells after 48 hrs by MTT assay	[PMID: 28792756]
MCF7	IC₅₀	48 nM Compound: isoCA-4	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 28792756]
MCF7	IC₅₀	8 nM Compound: isoCA-4	Antiproliferative activity against human MCF7 cells after 72 hrs by resazurin-based fluorescence assay Antiproliferative activity against human MCF7 cells after 72 hrs by resazurin-based fluorescence assay	[PMID: 29202409]
MCF7	GI₅₀	8 nM Compound: 3a; isoCA-4	Antiproliferative activity against human MCF7 cells after 72 hrs by MTS assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTS assay	[PMID: 30004697]
MCF7	IC₅₀	170 nM Compound: 3; isoCA4	Antiproliferative activity against human MCF7 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay Antiproliferative activity against human MCF7 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay	[PMID: 33038796]
MCF7	IC₅₀	8 nM Compound: isoCA4	Antiproliferative activity against human MCF7 cells after 72 hrs by CellTiter Glo assay Antiproliferative activity against human MCF7 cells after 72 hrs by CellTiter Glo assay	[PMID: 35797900]
MDA-MB-231	IC₅₀	4 nM Compound: isoCA-4	Cytotoxicity against human MDA-MB-231 cells after 72 hrs Cytotoxicity against human MDA-MB-231 cells after 72 hrs	[PMID: 19530698]
MDA-MB-231	GI₅₀	4 nM Compound: isoCA-4	Cytotoxicity against human MDA-MB-231 cells after 72 hrs using resazurin dye by microtiter plate fluorimeter analysis Cytotoxicity against human MDA-MB-231 cells after 72 hrs using resazurin dye by microtiter plate fluorimeter analysis	[PMID: 22449653]
MDA-MB-231	GI₅₀	4 nM Compound: 2a, isoCA-4	Growth inhibition of hormone-independent human MDA-MB-231 cells after 72 hrs by Victor microtiter plate fluorimeter analysis Growth inhibition of hormone-independent human MDA-MB-231 cells after 72 hrs by Victor microtiter plate fluorimeter analysis	[PMID: 23353744]
MDA-MB-231	GI₅₀	4.1 nM Compound: isoCA-4	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by CellTitre-Blue assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by CellTitre-Blue assay	[PMID: 25528337]
MDA-MB-231	IC₅₀	8.5 nM Compound: isoCA-4	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 28792756]
MDA-MB-231	IC₅₀	1.98 nM Compound: 3; isoCA-4	Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 34171660]
MDA-MB-231	IC₅₀	4 nM Compound: isoCA4	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by CellTiter Glo assay Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by CellTiter Glo assay	[PMID: 35797900]
MGC-803	IC₅₀	19.8 nM Compound: isoCA-4	Antiproliferative activity against human MGC803 cells after 48 hrs by MTT assay Antiproliferative activity against human MGC803 cells after 48 hrs by MTT assay	[PMID: 28792756]
MIA PaCa-2	GI₅₀	10 nM Compound: 3a; isoCA-4	Antiproliferative activity against human MIAPaCa2 cells after 72 hrs by MTS assay Antiproliferative activity against human MIAPaCa2 cells after 72 hrs by MTS assay	[PMID: 30004697]
MIA PaCa-2	IC₅₀	5 nM Compound: isoCA-4	Cytotoxicity against human MIAPaCa2 cells assessed as reduction in cell growth measured after 72 hrs by MTS assay Cytotoxicity against human MIAPaCa2 cells assessed as reduction in cell growth measured after 72 hrs by MTS assay	[PMID: 30818177]
MIA PaCa-2	IC₅₀	10 nM Compound: 3; isoCA4	Antiproliferative activity against human MiaPaca2 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay Antiproliferative activity against human MiaPaca2 cells assessed as cell viability after 24 hrs by Celltiter-Glo assay	[PMID: 33038796]
MIA PaCa-2	IC₅₀	3.01 nM Compound: 3; isoCA-4	Cytotoxicity against human MIA PaCa-2 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human MIA PaCa-2 cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 34171660]
MIA PaCa-2	IC₅₀	10 nM Compound: isoCA4	Antiproliferative activity against human MIA PaCa-2 cells after 72 hrs by CellTiter Glo assay Antiproliferative activity against human MIA PaCa-2 cells after 72 hrs by CellTiter Glo assay	[PMID: 35797900]
NCI-H1299	IC₅₀	5 nM Compound: isoCA-4	Cytotoxicity against human H1299 cells after 72 hrs Cytotoxicity against human H1299 cells after 72 hrs	[PMID: 19530698]
NCI-H1299	GI₅₀	3 nM Compound: isoCA-4	Cytotoxicity against human H1299 cells after 72 hrs using resazurin dye by microtiter plate fluorimeter analysis Cytotoxicity against human H1299 cells after 72 hrs using resazurin dye by microtiter plate fluorimeter analysis	[PMID: 22449653]
NCI-H1299	GI₅₀	3 nM Compound: 2a, isoCA-4	Growth inhibition of human H1299 cells after 72 hrs by Victor microtiter plate fluorimeter analysis Growth inhibition of human H1299 cells after 72 hrs by Victor microtiter plate fluorimeter analysis	[PMID: 23353744]
NCI-H1299	GI₅₀	5 nM Compound: isoCA-4	Cytotoxicity against human H1299 cells after 72 hrs by CellTitre-Blue assay Cytotoxicity against human H1299 cells after 72 hrs by CellTitre-Blue assay	[PMID: 25528337]
NCI-N87	IC₅₀	14 nM Compound: isoCA4	Antiproliferative activity against human NCI-N87 cells after 72 hrs by CellTiter Glo assay Antiproliferative activity against human NCI-N87 cells after 72 hrs by CellTiter Glo assay	[PMID: 35797900]
PBL	IC₅₀	1.7 μM Compound: 3a; isoCA-4	Cytotoxicity against human PBL cells assessed as cell growth inhibition after 72 hrs by MTS assay Cytotoxicity against human PBL cells assessed as cell growth inhibition after 72 hrs by MTS assay	[PMID: 30004697]
PC-3	IC₅₀	0.001 μM Compound: isoCA-4	Cytotoxicity against human PC3 cells assessed as reduction in cell viability incubated for 48 hrs by SRB assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability incubated for 48 hrs by SRB assay	[PMID: 28803799]
PC-3	GI₅₀	3 nM Compound: 3a; isoCA-4	Antiproliferative activity against human PC3 cells after 72 hrs by MTS assay Antiproliferative activity against human PC3 cells after 72 hrs by MTS assay	[PMID: 30004697]
RKO	IC₅₀	3.8 nM Compound: isoCA-4	Antiproliferative activity against human RKO cells after 48 hrs by MTT assay Antiproliferative activity against human RKO cells after 48 hrs by MTT assay	[PMID: 28792756]
SK-OV-3	IC₅₀	5 nM Compound: isoCA4	Antiproliferative activity against human SK-OV-3 cells after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SK-OV-3 cells after 72 hrs by CellTiter Glo assay	[PMID: 35797900]
U-87MG ATCC	IC₅₀	7.8 nM Compound: isoCA-4	Cytotoxicity against human U87 cells assessed as decrease in cell growth after 72 hrs by resazurin dye-based fluorimetric method Cytotoxicity against human U87 cells assessed as decrease in cell growth after 72 hrs by resazurin dye-based fluorimetric method	[PMID: 28166995]
U-87MG ATCC	IC₅₀	8 nM Compound: isoCA-4	Antiproliferative activity against human U87 cells after 72 hrs by resazurin-based fluorescence assay Antiproliferative activity against human U87 cells after 72 hrs by resazurin-based fluorescence assay	[PMID: 29202409]
U-87MG ATCC	GI₅₀	8 nM Compound: 3a; isoCA-4	Antiproliferative activity against human U87 cells after 72 hrs by MTS assay Antiproliferative activity against human U87 cells after 72 hrs by MTS assay	[PMID: 30004697]
U-87MG ATCC	IC₅₀	8.2 nM Compound: iso-CA-4	Antiproliferative activity against human U87MG cells after 72 hrs by MTS assay Antiproliferative activity against human U87MG cells after 72 hrs by MTS assay	[PMID: 30525602]
U-87MG ATCC	IC₅₀	8.24 nM Compound: 3; isoCA-4	Cytotoxicity against human U-87 MG cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human U-87 MG cells assessed as inhibition of cell growth measured after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 34171660]

Chemical Information

CAS No. 1067880-31-2
Molecular Weight 316.35
Formula C₁₈H₂₀O₅
SMILES

C=C(C1=CC(OC)=C(C(OC)=C1)OC)C2=CC(O)=C(C=C2)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (266 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Xiang-Yu Yan, et al. Design, synthesis, and biological evaluation of novel diphenylamine derivatives as tubulin polymerization inhibitors targeting the colchicine binding site. Eur J Med Chem. 2022 Apr 16;237:114372. [Content Brief]

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How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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