Phallacidin
Based on 1 Customer Validation
Phallacidin is a natural bicyclic heptapeptide derived from the poisonous mushroom Amanita phalloides. Phallacidin binds to filamentous actin specifically with high affinity, with a Kd of 20 nM. After binding to F-actin, Phallacidin strongly inhibits its depolymerization, stabilizes microfilament structures, and prevents their disruption by drugs such as cytochalasins. When conjugated with a fluorophore, Phallacidin serves as a specific fluorescent probe for F-actin, which is used to clearly visualize the distribution of actin in the cytoskeleton (e.g., stress fibers, cortical peripheral bands) under fluorescence microscopy.
For research use only. We do not sell to patients.
- Purity: 92.4%
- CAS No.: 26645-35-2
- Formula: C37H50N8O13S
- Molecular Weight:846.90
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Pretreatment with phallacidin (0.3 μM; 3-24 h) prevents α-thrombin-induced actin cytoskeletal rearrangements in cultured calf pulmonary artery endothelial monolayers[1].
Pretreatment with phallacidin (0.3 μM; 3-24 h) markedly attenuates α-thrombin-induced increases in 125I-albumin permeability across cultured calf pulmonary artery endothelial monolayers, without altering baseline permeability[1].
Phallacidin specifically labels polymerized F-actin in the kinetochore region of the mitotic spindle in Oedogonium spp. green algal cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 26645-35-2
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Appearance Solid
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Molecular Weight 846.90
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Formula C37H50N8O13S
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Color White to off-white
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Sequence
Ala-Trp-Leu-Val-{D-Asp}-Cys-{Hyp} (Sulfide bridge: Trp2- Cys6)
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Sequence Shortening
AWLV-{D-Asp}-C-{Hyp} (Sulfide bridge: Trp2- Cys6)
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Structure Classification
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Initial Source
Amanita exitialis basidiocarps
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
Purity & Documentation
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Data Sheet (259 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Handling Instructions (2659 KB)
References
[1]. Phillips PG, et al. Phallacidin prevents thrombin-induced increases in endothelial permeability to albumin. Am J Physiol. 1989;257(3 Pt 1):C562-C567. [Content Brief]
[2]. Sampson K, et al. Phallacidin stains the mitotic spindle of the diatom Pinnularia spp. Cell Biol Int. 2004;28(8-9):565-569. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Phallacidin Related Classifications
HY-P2031 Related Classifications
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