IXA6
Based on 1 Customer Validation
IXA6 is a novel IRE1/XBP1s activator, and can induce IRE1 RNase activity.
For research use only. We do not sell to patients.
- Purity: 99.29%
- CAS No.: 1021106-40-0
- Formula: C22H20ClN3O3S
- Molecular Weight:441.94
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
IXA6 (10 µM; 4 or 18 h) selectively activates IRE1-XBP1s signaling, and activates the XBP1s transcriptional response[1].
IXA6 (10 µM; 4 h) shows selectivity for IRE1-XBP1s-dependent ER proteostasis remodeling[1].
IXA6 (10 µM; 18 h) reduces secretion of APP through IRE1 activation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293T cells
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Concentration:10 µM
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Incubation Time:18 hours
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Result:Increasesd in ER proteostasis factor gene expression correspond to increased protein levels.
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Cell Line:HEK293T cells
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Concentration:10 µM
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Incubation Time:4 hours
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Result:Activated the IRE1-XBP1s geneset to levels about 30-40% that observed for Tg (Tg representing 100% activation of each gene).
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Cell Line:HEK293T cells
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Concentration:10 µM
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Incubation Time:4 hours
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Result:Observed high level of overlap (64%) for genes induced by XBP1s and IXA6.
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Cell Line:Huh7 and SHSY5Y cells
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Concentration:10 µM
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Incubation Time:4 hours
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Result:Upregulated XBP1s mRNA selectively, in cell lines including Huh7 and SHSY5Y cells.
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Cell Line:CHO cells
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Concentration:10 µM
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Incubation Time:18 hours
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Result:Observed reduction in Aβ secretion blocked by cotreatment with 4µ8c, confirming this reduction is dependent on IRE1 RNAse activity in CHO7WD10 cells stably expressing wild-type APP (APPWT) and in cells treated with the IXA6.
Chemical Information
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CAS No. 1021106-40-0
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Appearance Solid
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Molecular Weight 441.94
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Formula C22H20ClN3O3S
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Color White to light yellow
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SMILES
O=C(CN(S(=O)(C1=CC=CN=C1)=O)CC2=CC=C(C=C2)Cl)N3C4=C(CC3)C=CC=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 31.25 mg/mL (70.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.83 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2628 mL | 11.3138 mL | 22.6275 mL | 56.5688 mL |
| 5 mM | 0.4526 mL | 2.2628 mL | 4.5255 mL | 11.3138 mL | |
| 10 mM | 0.2263 mL | 1.1314 mL | 2.2628 mL | 5.6569 mL | |
| 15 mM | 0.1509 mL | 0.7543 mL | 1.5085 mL | 3.7713 mL | |
| 20 mM | 0.1131 mL | 0.5657 mL | 1.1314 mL | 2.8284 mL | |
| 25 mM | 0.0905 mL | 0.4526 mL | 0.9051 mL | 2.2628 mL | |
| 30 mM | 0.0754 mL | 0.3771 mL | 0.7543 mL | 1.8856 mL | |
| 40 mM | 0.0566 mL | 0.2828 mL | 0.5657 mL | 1.4142 mL | |
| 50 mM | 0.0453 mL | 0.2263 mL | 0.4526 mL | 1.1314 mL | |
| 60 mM | 0.0377 mL | 0.1886 mL | 0.3771 mL | 0.9428 mL |
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