1. Cell Cycle/DNA Damage
  2. IRE1
  3. IXA6

IXA6 is a novel IRE1/XBP1s activator, and can induce IRE1 RNase activity.

For research use only. We do not sell to patients.

IXA6 Chemical Structure

IXA6 Chemical Structure

CAS No. : 1021106-40-0

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5 mg USD 540 In-stock
10 mg USD 864 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

IXA6 is a novel IRE1/XBP1s activator, and can induce IRE1 RNase activity[1].

In Vitro

IXA6 (10 µM; 4 or 18 h) selectively activates IRE1-XBP1s signaling, and activates the XBP1s transcriptional response[1].
IXA6 (10 µM; 4 h) shows selectivity for IRE1-XBP1s-dependent ER proteostasis remodeling[1].
IXA6 (10 µM; 18 h) reduces secretion of APP through IRE1 activation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HEK293T cells
Concentration: 10 µM
Incubation Time: 18 hours
Result: Increasesd in ER proteostasis factor gene expression correspond to increased protein levels.

RT-PCR[1]

Cell Line: HEK293T cells
Concentration: 10 µM
Incubation Time: 4 hours
Result: Activated the IRE1-XBP1s geneset to levels about 30-40% that observed for Tg (Tg representing 100% activation of each gene).

Cell Viability Assay[1]

Cell Line: HEK293T cells
Concentration: 10 µM
Incubation Time: 4 hours
Result: Observed high level of overlap (64%) for genes induced by XBP1s and IXA6.

RT-PCR[1]

Cell Line: Huh7 and SHSY5Y cells
Concentration: 10 µM
Incubation Time: 4 hours
Result: Upregulated XBP1s mRNA selectively, in cell lines including Huh7 and SHSY5Y cells.

RT-PCR[1]

Cell Line: CHO cells
Concentration: 10 µM
Incubation Time: 18 hours
Result: Observed reduction in Aβ secretion blocked by cotreatment with 4µ8c, confirming this reduction is dependent on IRE1 RNAse activity in CHO7WD10 cells stably expressing wild-type APP (APPWT) and in cells treated with the IXA6.
Molecular Weight

441.93

Formula

C22H20ClN3O3S

CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(CN(S(=O)(C1=CC=CN=C1)=O)CC2=CC=C(C=C2)Cl)N3C4=C(CC3)C=CC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (75.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2628 mL 11.3140 mL 22.6280 mL
5 mM 0.4526 mL 2.2628 mL 4.5256 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.83 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.29%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2628 mL 11.3140 mL 22.6280 mL 56.5700 mL
5 mM 0.4526 mL 2.2628 mL 4.5256 mL 11.3140 mL
10 mM 0.2263 mL 1.1314 mL 2.2628 mL 5.6570 mL
15 mM 0.1509 mL 0.7543 mL 1.5085 mL 3.7713 mL
20 mM 0.1131 mL 0.5657 mL 1.1314 mL 2.8285 mL
25 mM 0.0905 mL 0.4526 mL 0.9051 mL 2.2628 mL
30 mM 0.0754 mL 0.3771 mL 0.7543 mL 1.8857 mL
40 mM 0.0566 mL 0.2829 mL 0.5657 mL 1.4143 mL
50 mM 0.0453 mL 0.2263 mL 0.4526 mL 1.1314 mL
60 mM 0.0377 mL 0.1886 mL 0.3771 mL 0.9428 mL
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IXA6 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
IXA6
Cat. No.:
HY-139212
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