JF630-HTL
JF630-HTL is a fluorogenic Janelia Fluor (JF) Si-rhodamine HaloTag ligand. JF630-HTL covalently labels HaloTag fusion proteins in living cells. JF630-HTL displays low fluorescence in the unbound state, but exhibits a marked fluorescence enhancement upon binding to HaloTag. JF630-HTL can be used for live-cell imaging applications.
For research use only. We do not sell to patients.
- Formula: C43H54ClNO9S2Si
- Molecular Weight:856.56
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
JF630-HTL produces a 7.0-fold reversible deep-red fluorescence turn-on upon 450 nm illumination in live U2OS cells expressing LifeAct-targeted psHaloTag1a[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Molecular Weight 856.56
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Formula C43H54ClNO9S2Si
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SMILES
ClCCCCCCOCCOCCNC(C(C=C1)=CC(C23C4=CC=C(C5CC(S(=O)(C)=O)C5)C=C4[Si](C)(C)C6=C2C=CC(C7CC(S(=O)(C)=O)C7)=C6)=C1C(O3)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)