バソフェナントロリン
Based on 1 Customer Validation
Bathophenanthroline is a nitrogen-containing organic ligand that can form stable complexes with transition metal ions such as Ru2+. In proteomics, Bathophenanthroline is often used for in-gel protein staining. The negatively charged sulfonic acid groups of Bathophenanthroline interact electrostatically with the positively charged amino acid residues of proteins, thereby achieving specific binding to proteins and enhancing the fluorescent signal of detection. Bathophenanthroline can be used for protein separation and detection in SDS-PAGE gels in the field of proteomics, as well as other fluorescence studies[1]. Ex/Em=532 nm/610 nm[1].
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.73%
- CAS 番号: 1662-01-7
- 分子式: C24H16N2
- 分子量:332.40
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保管条件:
RT, protect from light, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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HY-D0005
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Dansyl chlorideFluorescent Dye -
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Bathocuproine disulfonate disodiumBiochemical Assay Reagent -
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NBD-ClNonfluorescent Reagent -
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Oil Red ODiazol Dye -
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Lead (II) iodideChemical Compound -
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4-Nitrophenyl phosphate disodiumPhosphatases Substrate -
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9-AminoacridineAntimicrobial
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Huh-7 | CC50 |
>100 μM
Compound: GNF-Pf-4554
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NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
|
[PMID: 18579783] |
体外実験
In SDS-PAGE gel staining experiments, heteroligand ruthenium(II) complexes containing bathophenanthroline (BP) and bathophenanthroline disulfonate (BPS) shows higher sensitivity than SYPRO Ruby for protein detection at near-neutral pH and reduces artificial post-translational modifications[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Fix gels in 7% acetic acid/10% ethanol.
2. Stain overnight with 0.5-1 mg/L Ru(BPS)2(BP) or Ru(BPS)(BP)2 in acidic/neutral buffer, destained.
3. Scan for fluorescence at 532 nm excitation/610 nm emission.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 1662-01-7
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性状 Solid
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分子量 332.40
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分子式 C24H16N2
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Color Off-white to light yellow
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SMILES
C12=C(N=CC=C3C4=CC=CC=C4)C3=CC=C1C(C5=CC=CC=C5)=CC=N2
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別名
Bathophenanthroline
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
RT, protect from light, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
溶剤 & 溶解度
体外:
DMSO : 2.78 mg/mL (8.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (275 KB)
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SDS (396 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0084 mL | 15.0421 mL | 30.0842 mL | 75.2106 mL |
| 5 mM | 0.6017 mL | 3.0084 mL | 6.0168 mL | 15.0421 mL |
Bathophenanthroline 関連分類
HY-D0006 関連分類
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