DAF-2DA
Based on 2 publication(s) in Google Scholar
DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 95.70%
- CAS 番号: 205391-02-2
- 分子式: C24H18N2O7
- 分子量:446.41
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保管条件:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
MedChemExpress(MCE)の使用を引用している文献 DAF-2DA
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生物活性
Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF-2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF-2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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化学情報
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CAS 番号 205391-02-2
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性状 Solid
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分子量 446.41
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分子式 C24H18N2O7
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Color Light yellow to yellow
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SMILES
O=C1OC2(C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3)C5=C1C=C(N)C(N)=C5
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別名
5,6-Diaminofluorescein diacetat
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell Metab
Lighting up arginine metabolism reveals its functional diversity in physiology and pathology. [Abstract]2025 Jan 7;37(1):291-304.e9. PMID: 39413790 -
Cell Commun Signal
Klebsiella pneumoniae-derived extracellular vesicles impair endothelial function by inhibiting SIRT1. [Abstract]2025 Jan 13;23(1):21. PMID: 39800699
純度とドキュメンテーション
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データシート (272 KB)
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SDS (558 KB)
- English - EN (558 KB)
- Français - FR (558 KB)
- Deutsch - DE (558 KB)
- Norwegian - NO (558 KB)
- Español - ES (558 KB)
- Swedish - SV (558 KB)
- Italian - IT (558 KB)
- Korean - KR (558 KB)
- Portuguese - PT (558 KB)
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取扱説明書 (2659 KB)
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)