Dansylamide
Based on 1 Customer Validation
Dansyl amide is a fluorescent dye that is used in biochemistry and chemistry to label substances with the fluorescent dansyl group (Ex/Em: 360 nm/465 nm).
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.28%
- CAS 番号: 1431-39-6
- 分子式: C12H14N2O2S
- 分子量:250.32
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保管条件:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
生物活性
製品説明
体外実験
Dansylamide (DNSA), a site I specific probe, shows a significant increase in the fluorescence intensity of S-homocysteinylated rHSA (Hcys-rHSA)[2].
For the analysis of the binding ability of albumin, HSAs (10 μM) and BD140 (3 μM) or Dansylamide (DNSA) (10 μM) are incubated in phosphate buffer (pH 7.3) at room temperature, and fluorescence measurements are taken after incubation for 30 min[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
554
Excitation (Ex)
340
化学情報
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CAS 番号 1431-39-6
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性状 Solid
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分子量 250.32
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分子式 C12H14N2O2S
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Color White to yellow
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SMILES
O=S(C1=C2C=CC=C(C2=CC=C1)N(C)C)(N)=O
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別名
ダンシルアミド
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (399.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Misra A, et al. Fluorescent labelling of ribonucleosides at 2'-terminus; comparative fluorescence studies. Nucleic Acids Symp Ser. 2000;(44):291-2. [Content Brief]
[2]. Nakashima F, et al. Structural and functional insights into S-thiolation of human serum albumins. Sci Rep. 2018 Jan 17;8(1):932. [Content Brief]
[3]. Tanaka Y, et al. 8-Prenylnaringenin tissue distribution and pharmacokinetics in mice and its binding to human serum albumin and cellular uptake in human embryonic kidney cells. Food Sci Nutr. 2022 Jan 22;10(4):1070-1080. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.9949 mL | 19.9744 mL | 39.9489 mL | 99.8722 mL |
| 5 mM | 0.7990 mL | 3.9949 mL | 7.9898 mL | 19.9744 mL | |
| 10 mM | 0.3995 mL | 1.9974 mL | 3.9949 mL | 9.9872 mL | |
| 15 mM | 0.2663 mL | 1.3316 mL | 2.6633 mL | 6.6581 mL | |
| 20 mM | 0.1997 mL | 0.9987 mL | 1.9974 mL | 4.9936 mL | |
| 25 mM | 0.1598 mL | 0.7990 mL | 1.5980 mL | 3.9949 mL | |
| 30 mM | 0.1332 mL | 0.6658 mL | 1.3316 mL | 3.3291 mL | |
| 40 mM | 0.0999 mL | 0.4994 mL | 0.9987 mL | 2.4968 mL | |
| 50 mM | 0.0799 mL | 0.3995 mL | 0.7990 mL | 1.9974 mL | |
| 60 mM | 0.0666 mL | 0.3329 mL | 0.6658 mL | 1.6645 mL | |
| 80 mM | 0.0499 mL | 0.2497 mL | 0.4994 mL | 1.2484 mL | |
| 100 mM | 0.0399 mL | 0.1997 mL | 0.3995 mL | 0.9987 mL |