SPQ
Based on 1 Customer Validation
SPQ is a fluorescent dye. SPQ can be used to measure chloride ion concentration transients and transport rates in vesicles, cells, and even intact epithelium.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.82%
- CAS 番号: 83907-40-8
- 分子式: C13H15NO4S
- 分子量:281.33
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
生物活性
SPQ (1 mM or 10 mM, 16 h in 4°C or 30 min 37°C) can accurately and real-time report changes in intracellular Cl- concentration in human erythrocyte ghosts[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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化学情報
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CAS 番号 83907-40-8
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性状 Solid
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分子量 281.33
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分子式 C13H15NO4S
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Color White to gray
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SMILES
O=S(CCC[N+]1=C2C=CC(OC)=CC2=CC=C1)([O-])=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
H2O : 25 mg/mL (88.86 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (273 KB)
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SDS (393 KB)
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- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Sermet-Gaudelus I, et al. Normal function of the cystic fibrosis conductance regulator protein can be associated with homozygous (Delta)F508 mutation. Pediatr Res. 2002 Nov;52(5):628-35. [Content Brief]
[2]. Illsley NP, et al. Membrane chloride transport measured using a chloride-sensitive fluorescent probe. Biochemistry. 1987 Mar 10;26(5):1215-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.5545 mL | 17.7727 mL | 35.5454 mL | 88.8636 mL |
| 5 mM | 0.7109 mL | 3.5545 mL | 7.1091 mL | 17.7727 mL | |
| 10 mM | 0.3555 mL | 1.7773 mL | 3.5545 mL | 8.8864 mL | |
| 15 mM | 0.2370 mL | 1.1848 mL | 2.3697 mL | 5.9242 mL | |
| 20 mM | 0.1777 mL | 0.8886 mL | 1.7773 mL | 4.4432 mL | |
| 25 mM | 0.1422 mL | 0.7109 mL | 1.4218 mL | 3.5545 mL | |
| 30 mM | 0.1185 mL | 0.5924 mL | 1.1848 mL | 2.9621 mL | |
| 40 mM | 0.0889 mL | 0.4443 mL | 0.8886 mL | 2.2216 mL | |
| 50 mM | 0.0711 mL | 0.3555 mL | 0.7109 mL | 1.7773 mL | |
| 60 mM | 0.0592 mL | 0.2962 mL | 0.5924 mL | 1.4811 mL | |
| 80 mM | 0.0444 mL | 0.2222 mL | 0.4443 mL | 1.1108 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.