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製品番号: HY-D3423

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AM2-10

Name: AM2-10
Brand: MedChemExpress
SKU: HY-D3423
Availability: PreOrder

(別名: FM 2-10FX)

AM2-10 (FM 2-10FX) is a Fluorescent dye used for liquid-phase endocytosis imaging. AM2-10 stains cell membranes to observe neurite length during NGF-induced cell differentiation.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

分子式: C₂₅H₄₁Cl₃N₄
分子量:503.98

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

AM2-10 構造式

容量

価格（税別）

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Amount: USD 0.00

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

AM2-10 (FM 2-10FX) is a Fluorescent dye used for liquid-phase endocytosis imaging. AM2-10 stains cell membranes to observe neurite length during NGF-induced cell differentiation^[1].

体外実験

Guide (The following is our recommended protocol. This protocol is for guidance only and should be modified according to your specific needs).

1. Stock Solution Preparation
1.1 Solvent: OptiMEM.
1.2 Concentration recommendation: 20 μM.

3. Staining Procedure
3.1 Sample type: Adherent cells (PC12 cells grown on poly-L-lysine coverslips).[1]
3.2 Incubation conditions: Incubate cells with 20 μM AM2-10 for 5 min at 37°C; or image cells in solution containing AM2-10 for NGF-induced differentiation assessment.
3.3 Washing steps: Wash cells twice with ice-cold PBS.

5. Detection & Analysis
5.1 Instrument: Fluorescence microscope.
5.2 Result analysis:
5.2.1 Fluorescence localization: Stains all cellular membranes, including endocytic vesicles and neurite membranes.
5.2.2 Result application: Used to assess fluid-phase endocytosis efficiency and NGF-induced neurite differentiation in PC12 cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

化学情報

分子量 503.98
分子式 C₂₅H₄₁Cl₃N₄
SMILES

CCN(CC)C1=CC=C(/C=C/C2=CC=[N+](CCC[N+](C)(C)CCC[NH3+])C=C2)C=C1.[Cl-].[Cl-].[Cl-]
別名

FM 2-10FX
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Bethani I, et al. Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking. Traffic (Copenhagen, Denmark). 2009 Oct;10(10):1543-59.

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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