AMT-NHS
Based on 1 Customer Validation
AMT-NHS is an RNA-protein crosslinker. AMT-NHS consists of a psoralen derivative and an N-hydroxysuccinimide ester group, which react with RNA bases and primary amines of proteins, respectively. AMT-NHS penetrates live yeast cells and crosslinks Cbf5 to H/ACA snoRNAs with high specificity. AMT-NHS induces distinct crosslinking patterns and targets both single-stranded and double-stranded regions of RNA. AMT-NHS can be used to capture various RNA-protein interactions in cells.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.70%
- CAS 番号: 2925268-86-4
- 分子式: C22H21NO7S
- 分子量:443.47
-
保管条件:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
製品説明
体外実験
AMT-NHS (0.1-1.5 mM; incubated in the dark for 30 min and irradiated for 30 min) penetrates live Saccharomyces cerevisiae cells and crosslinks Cbf5 to H/ACA snoRNA, and the H/ACA snoRNA readout specificity increases with increasing concentration[1].
AMT-NHS induces significant nonspecific crosslinking of Rnt1 with highly abundant tRNA and rRNA in yeast[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 2925268-86-4
-
性状 Solid
-
分子量 443.47
-
分子式 C22H21NO7S
-
Color White to off-white
-
SMILES
O=C(ON1C(CCC1=O)=O)CCSCC2=C(C)OC(C(C)=C3O4)=C2C=C3C(C)=CC4=O
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
体外:
DMSO : 25 mg/mL (56.37 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
-
データシート (273 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2549 mL | 11.2747 mL | 22.5494 mL | 56.3736 mL |
| 5 mM | 0.4510 mL | 2.2549 mL | 4.5099 mL | 11.2747 mL | |
| 10 mM | 0.2255 mL | 1.1275 mL | 2.2549 mL | 5.6374 mL | |
| 15 mM | 0.1503 mL | 0.7516 mL | 1.5033 mL | 3.7582 mL | |
| 20 mM | 0.1127 mL | 0.5637 mL | 1.1275 mL | 2.8187 mL | |
| 25 mM | 0.0902 mL | 0.4510 mL | 0.9020 mL | 2.2549 mL | |
| 30 mM | 0.0752 mL | 0.3758 mL | 0.7516 mL | 1.8791 mL | |
| 40 mM | 0.0564 mL | 0.2819 mL | 0.5637 mL | 1.4093 mL | |
| 50 mM | 0.0451 mL | 0.2255 mL | 0.4510 mL | 1.1275 mL |
AMT-NHS 関連分類
HY-150014 関連分類
Powered by Bioz