Arcopilin A
Arcopilin A (compound Arcopilin A(1))is an antibacterial agent. Arcopilin A has weak inhibitory effects on fungal pathogens and Gram-positive bacteria, with IC50 values of 8.9 μg/mL and 14 μg/mL for cells KB-3-1 and L929, but it can effectively destroy preformed biofilms of Staphylococcus aureus. Arcopilin A can enhance the activities of gentamicin (GM; HY-K1050) and vancomycin (Vac; HY-B0671) by 115 and 31 times, respectively.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C22H27NO4
- 分子量:369.45
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| KB 3-1 | IC50 |
8.9 μg/mL
Compound: 1; Acp A
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Cytotoxicity against human KB-3-1 cells assessed as inhibition of cell growth incubated for 5 days by MTT assay
Cytotoxicity against human KB-3-1 cells assessed as inhibition of cell growth incubated for 5 days by MTT assay
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[PMID: 39141525] |
| L929 | IC50 |
14 μg/mL
Compound: 1; Acp A
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Cytotoxicity against mouse L929 cells assessed as inhibition of cell growth incubated for 5 days by MTT assay
Cytotoxicity against mouse L929 cells assessed as inhibition of cell growth incubated for 5 days by MTT assay
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[PMID: 39141525] |
体外実験
Arcopilin A disruptes preformed biofilms by approximately 50-60% in the concentration range of 15.6-250 μg/mL, and can also inhibit the growth of Staphylococcus aureus at concentrations as low as 3.9 and 7.8 μg/mL[1].
Arcopilin A has an MIC value of 66.7 μg/mL against B.subtilis DSM 10, S.aureus DSM 346, S.pombe DSM70572, C.albicans DSM 1665, and M.hiemalis DSM 2656, and an MIC value of 31.3 μg/mL against S.aureus DSM 1104[1].
Arcopilin A (3.9-31.1 μg/mL) reduces biofilm metabolic activity in a dose-dependent manner[1].
Arcopilin A (2-7.8 μg/mL) inhibits the growth of Staphylococcus aureus DSM 1104 planktonic cells[1].
Arcopilin A (3.9-7.8 μg/mL, 24 h) when used with GM (HY-K1050) and Vac (HY-B0671) have a synergistic effect on Staphylococcus aureus DSM 1104 planktonic cells. The MIC values of the established antibiotics were reduced from 15.6 to 0.13 μg/mL and from 2 to 0.065 μg/mL respectively. The combined treatment significantly increased the potency of GM (HY-K1050) and Vac (HY-B0671) by 115-fold and 31-fold, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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分子量 369.45
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分子式 C22H27NO4
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SMILES
CC([C@@H](C[C@H](C[C@@H](C(C1=C(/C(NC1=O)=C/C2=CC=CC=C2)O)=O)C)C)C)=O
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Structure Classification
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Initial Source
Arcopilus navicularis CCF 3252T
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Arcopilin A 関連分類
HY-N13196 関連分類
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