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製品番号: HY-D1931

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ATTO 590

ATTO 590 is a rhodamine-class fluorescent dye that can be used for labeling DNA, RNA, or proteins. ATTO 590 features strong absorption, high fluorescence quantum yield, and excellent thermal and photostability. ATTO 590 can be used in various technical detections such as single-molecule detection, flow cytometry (FACS), and fluorescence in situ hybridization (FISH) (λabs/λem = 593-594/622-624 nM).

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 639065-13-7
分子式: C₃₇H₃₉ClN₂O₉
分子量:691.17

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (271 KB)

ATTO 590 構造式

CAS 番号: 639065-13-7

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

ATTO 590 is used as a single-molecule sample or label on the surface of nanobeads, and attached to the microtubule network of fixed PtK2 cells via immunolabeling, for imaging validation of polarization-modulated super-resolution microscopy (SPoD/ExPAN)^[2].
ATTO 590-siRNA conjugates can be used for FRET live-cell imaging in rat brain endothelial cells (RBE4) to track the intracellular uptake and degradation of siRNA^[1].
ATTO 590 complexed with oligonucleotides can be used to label open chromatin in fixed mouse hippocampal CA1 pyramidal neuron tissue sections (ATAC-see technique)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

化学情報

CAS 番号 639065-13-7
分子量 691.17
分子式 C₃₇H₃₉ClN₂O₉
SMILES

CC1=CC(C)(C)N(CC)C2=C1C=C(C(C3=CC=CC=C3C(O)=O)=C(C=C(C(C)=CC(C)(C)N4CC)C4=C5)C5=[O+]6)C6=C2.O=Cl(=O)([O-])=O.CC(O)=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (271 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Hirsch M, et al. Live cell imaging of duplex siRNA intracellular trafficking. Nucleic acids research. 2015 May 19;43(9):4650-60.

[2]. Hafi N, et al. Fluorescence nanoscopy by polarization modulation and polarization angle narrowing. Nature methods. 2014 May;11(5):579-84.

[3]. Meyer SA, et al. Super-resolution imaging of ciliary microdomains in isolated olfactory sensory neurons using a custom two-color stimulated emission depletion microscope. Journal of biomedical optics. 2016 Jun 01;21(6):66017.

[4]. Sheu SH, et al. A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility. Cell. 2022 Sep 01;185(18):3390-3407.e18.

[5]. Kumar SR, et al. TLR9-independent CD8+ T cell responses in hepatic AAV gene transfer through IL-1R1-MyD88 signaling. Molecular Therapy. 2024 Feb 7;32(2):325-39. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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