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製品番号: HY-D3404

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BODi-1

BODi-1 is a fluorescent modulator targeting dsDNA, which binds to dsDNA via a bis-intercalation mechanism (E_x=465 nm, E_m=490 nm). BODi-1 exhibits a fluorescence enhancement effect upon binding to nucleic acids, but its fluorescence intensity, anisotropy and average lifetime decrease at higher dye/DNA ratios. When BODi-1 binds to DNA in liposome complexes, it also shows red-shifted emission spectra, along with reduced quantum yield and average lifetime. BODi-1 does not induce significant DNA conformational changes when the dye/DNA ratio is below 0.01. BODi-1 can be used as a fluorescent probe for the characterization of liposome complexes and FRET studies at this ratio.

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CAS 番号: 169454-13-1
分子式: C₄₁H₅₄I₄N₆S₂
分子量:1202.66

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

BODi-1 構造式

CAS 番号: 169454-13-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

BOBO-1 exhibits stable fluorescence intensity and anisotropy (~0.20) in supercoiled and relaxed pUC19 DNA when the dye/DNA base ratio ≤ 0.01^[1].
BOBO-1 maintains stable fluorescence anisotropy (~0.20) in pUC19 DNA/DOTAP lipid complexes when the dye/DNA base ratio is ≤0.005; at higher ratios, its fluorescence intensity and anisotropy decrease^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

化学情報

CAS 番号 169454-13-1
分子量 1202.66
分子式 C₄₁H₅₄I₄N₆S₂
SMILES

C[N+]1=C(/C=C2C=CN(CCC[N+](C)(C)CCC[N+](C)(C)CCCN3C=C/C(C=C3)=C\C4=[N+](C)C5=C(C=CC=C5)S4)C=C\2)SC6=C1C=CC=C6.[I-].[I-].[I-].[I-]
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Madeira C, et al. Photophysical behavior of a dimeric cyanine dye (BOBO-1) within cationic liposomes. Photochem Photobiol. 2005;81(6):1450-1459. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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