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Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Bromophenol blue is used as a sensor for the determination of several compounds including ammonia, drugs, proteins and amino acids. The binding of Bromophenol blue to proteins is accompanied by the appearance of a strong absorbance at 610 nm.
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- CAS 番号: 115-39-9
- 分子式: C19H10Br4O5S
- 分子量:669.96
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保管条件:
RT, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of Bromophenol blue Staining Solution:[2]
Prepare Bromophenol blue staining solution with DMSO.
2. Protein assay:[2]
2.1 Proteins were dissolved in distilled water, and their concentrations were adjusted to 1 mg/mL with the exceptions of the myoglobin solution which was prepared gravimetrically, and the y-globulin solution, which was made according to the suppliers.
2.2 For performing the protein assay, protein solutions containing from 10 to 80 μg of protein in 0.1 mL were mixed with 0.9 mL of the Bromophenol blue solution.
2.3 The absorbance at 610 nm was determined using a blank made by mixing 0.1 mL of distilled water and 0.9 mL of the dye solution.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 115-39-9
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性状 Solid
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分子量 669.96
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分子式 C19H10Br4O5S
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Color Orange to red
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SMILES
O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC=CC=C41)=O
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別名
Bromophenol blue
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
RT, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
溶剤 & 溶解度
DMSO : 50 mg/mL (74.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (282 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Dangui AZ, et, al. Preferential solvation bromophenol blue in water-alcohol binary mixture. Spectrochim Acta A Mol Biomol Spectrosc. 2018 Oct 5; 203: 333-341. [Content Brief]
[2]. Flores R. A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue. Anal Biochem. 1978 Aug 1;88(2):605-11. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4926 mL | 7.4631 mL | 14.9263 mL | 37.3157 mL |
| 5 mM | 0.2985 mL | 1.4926 mL | 2.9853 mL | 7.4631 mL | |
| 10 mM | 0.1493 mL | 0.7463 mL | 1.4926 mL | 3.7316 mL | |
| 15 mM | 0.0995 mL | 0.4975 mL | 0.9951 mL | 2.4877 mL | |
| 20 mM | 0.0746 mL | 0.3732 mL | 0.7463 mL | 1.8658 mL | |
| 25 mM | 0.0597 mL | 0.2985 mL | 0.5971 mL | 1.4926 mL | |
| 30 mM | 0.0498 mL | 0.2488 mL | 0.4975 mL | 1.2439 mL | |
| 40 mM | 0.0373 mL | 0.1866 mL | 0.3732 mL | 0.9329 mL | |
| 50 mM | 0.0299 mL | 0.1493 mL | 0.2985 mL | 0.7463 mL | |
| 60 mM | 0.0249 mL | 0.1244 mL | 0.2488 mL | 0.6219 mL |
Bromophenol blue 関連分類
HY-B1571 関連分類
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