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製品番号: HY-179648

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CD28-IN-2

CD28-IN-2 is a selective CD28-B7 interaction (IC₅₀: 22.4 μM) inhibitor that binds directly to CD28, with a K_d value of 24.1 μM. CD28-IN-2 inhibits CD28-driven immune activation and blocks T cell costimulation. CD28-IN-2 can be used for the study of antitumor immunity and immune-related disorders.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 1240857-57-1
分子式: C₁₉H₁₇FN₂O₃
分子量:340.35

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

CD28-IN-2 構造式

CAS 番号: 1240857-57-1

容量

価格（税別）

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数量

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

CD28-IN-2 (5MS-5) (10-50 μM; 48 hours) effectively inhibits CD28-dependent cytokine release and T cell activation markers (such as IFN-γ, IL-2,and soluble CD69 (sCD69)) in a 3D coculture model comprising IFN-γ-primed A549 tumor spheroids and human PBMCs^[1].
In CD28-expressing Jurkat T cells containing a TCR/CD3-CD28-responsive luciferase reporter are cocultured with Raji antigen-presenting cells, CD28-IN-2 exhibits dose-dependent inhibition of CD28-mediated luminescence, with an IC₅₀ of 10.3 μM^[1].
CD28-IN-2 shows a weak inhibition of the hERG potassium channel (IC₅₀ > 50 μM). CD28-IN-2 against major cytochrome P450 isoforms revealed minimal inhibition at 10 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[1]

Cell Line:

A 3D coculture model comprising IFN-γ-primed A549 tumor spheroids and human PBMCs.
Concentration:

10 μM, 25 μM, 50 μM
Incubation Time:

48 h
Result:

Resulted in concentration-dependent suppression of Tcell activation, indicating effective inhibition of CD28-mediated costimulation.

Parmacokinetics

Species	Dose	Route	C_max	AUC_0-t	T_1/2	T_max	CL	V_d
Mice^[1]	2 mg/kg	i.v.	4,440 ng/mL	10,392 ng·h/mL	1.62 h	/	3.21 mL/min/kg	0.45 L/kg
Mice^[1]	10 mg/kg	p.o.	3,024 ng/mL	9,401 ng·h/mL	2.55 h	1.12 h	/	/

化学情報

CAS 番号 1240857-57-1
分子量 340.35
分子式 C₁₉H₁₇FN₂O₃
SMILES

COC1=CC=C(CC(OCC2=CC=C(C=C2)N3C=CC=N3)=O)C=C1F
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (269 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Upadhyay S, et al. Discovery of CD28-Targeted Small Molecule Inhibitors of T Cell Co-Stimulation Using Affinity Selection-Mass Spectrometry (AS-MS) and Ex Vivo Validation. J Med Chem. 2025;68(23):25112-25125. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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