CHVB-066
CHVB-066 is an alphavirus non-structural protein nsP1 inhibitor that exhibits varying degrees of inhibitory activity against nsP1 of CHIKV and VEEV. CHVB-066 inhibits MTase and GTase activities and disrupts the capping process of viral RNA, including inhibiting CHIKV replication, reducing progeny viruses, and preventing cytopathic effects. It shows varying degrees of inhibitory activity against nsP1 of CHIKV and VEEV but lacks anti-SFV replication activity, displays cross-resistance with MADTP series compounds, and requires nsP2/nsP3 mutations to develop complete drug resistance. This inhibitor with varying inhibitory activity against nsP1 of CHIKV and VEEV can be widely applied to relevant studies on chikungunya virus infection and chikungunya fever.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 1309199-94-7
- 分子式: C19H25N5O
- 分子量:339.43
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Vero | CC50 |
203 μM
Compound: 31f
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Cytotoxicity against African green monkey Vero cells assessed as reduction in overall metabolic activity incubated for 5 days by MTS reagent based absorbance method
Cytotoxicity against African green monkey Vero cells assessed as reduction in overall metabolic activity incubated for 5 days by MTS reagent based absorbance method
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[PMID: 38104375] |
体外実験
CHVB-066 (Serial dilutions; 96 h) potently inhibits CHIKV replication in Vero E6 cells with an EC50 of 0.6 μM and has a CC50 of 25 μM, with no activity against SFV[1].
CHVB-066 (0.5-32 μM; 30 min) dose-dependently inhibits the combined methyltransferase and guanylyltransferase enzymatic activity of purified wild-type CHIKV nsP1 at concentrations ranging from 0.5 to 32 μM[1].
CHVB-066 (0.16-5.00 μM; 48 h) induces a dose-dependent reduction in CHIKV 899 viral RNA and infectious progeny production in Vero cells, with a significant >4-log10 reduction at 5.00 μM after 48 h[2].
CHVB-066 (0-12.5 μM; 4 days) inhibits wild-type rCHIKV LS3 replication in Vero E6 cells with an EC50 of 0.65 μM; resistance requires the combined nsP1S454G and nsP1W456R mutations, with maximum resistance (18.5-fold) achieved when combined with nsP2M703T and nsP3L494P mutations[2].
CHVB-066 (0.5-1000 μM) potently inhibits the guanylyltransferase activity of purified VEEV nsP1 with an IC50 of <0.5 μM[2].
CHVB-066 (0.001-100 μM) inhibits the methyltransferase activity of purified VEEV nsP1 with an IC50 of 1.9 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Vero E6 cells
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Concentration:Serial dilutions
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Incubation Time:96 h
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Result:Inhibited CHIKV-induced CPE with a 50% effective concentration (EC50) of 0.6 μM.
Exhibited a 50% cytotoxic concentration (CC50) of 25 μM.
Showed no antiviral activity against Semliki Forest virus (SFV).\nRendered CHIKV nsP1-P34S and CHIKV nsP1-S454G+W456R mutants completely resistant, with an EC50 of >12.5 μM and a fold resistance of 20.8 compared to wild-type CHIKV.
Maintained activity against CHIKV nsP1-G230R+K299E mutant, with an EC50 of 0.3 μM and a fold resistance <1.
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Cell Line:Vero cells
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Concentration:0.16-5.00 μM
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Incubation Time:48 h
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Result:Caused a marked dose-dependent reduction in CHIKV 899 viral genome copies and infectious virus progeny.
Induced a significant >4-log10 reduction in both viral RNA and infectious virus yield at 5.00 μM compared to untreated controls.
化学情報
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CAS 番号 1309199-94-7
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分子量 339.43
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分子式 C19H25N5O
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SMILES
O=C(N1CCN(CC1)C2=NC(NCC)=NC(C)=C2)C3=CC=C(C)C=C3
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Besztercei B, et al. Three nitrogen atoms turn old kinase inhibitors into new targetable remedy[J]. bioRxiv, 2026: 2026.04. 11.717649. [Content Brief]
[2]. Abdelnabi R, Kovacikova K, Moesslacher J, et al. Novel class of chikungunya virus small molecule inhibitors that targets the viral capping machinery[J]. Antimicrobial Agents and Chemotherapy, 2020, 64(7): 10.1128/aac. 00649-20. [Content Brief]
[4]. Kovacikova K, van Hemert M J. Small-molecule inhibitors of chikungunya virus: mechanisms of action and antiviral drug resistance[J]. Antimicrobial agents and chemotherapy, 2020, 64(12): 10.1128/aac. 01788-20. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
CHVB-066 関連分類
HY-184264 関連分類
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