DAz-2
Based on 1 Customer Validation
DAz-2 is a sulfonic acid probe for living cells, which can directly detect sulfonic acid-modified proteins in living cells and is cell-permeable.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.9%
- CAS 番号: 1176905-54-6
- 分子式: C9H13N3O2
- 分子量:195.22
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保管条件:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
This experimental protocol is designed to identify protein oxidation in living cells.
1. Cell labeling (live cells):
1.1 Incubate the cells (such as Jurkat cells) with DAz-2 (1-10 mM) in a culture medium containing 2% FBS at 37°C for 1-2 hours.
1.2 Wash the cells three times with PBS to remove the excess probes.
1.3 Lysis: Prepare the cell lysis buffer (for example, using RIPA or an equivalent lysis solution).
2. Bioorthogonal Labeling :
2.1 Incubate the lysate with 100 μM p-biotin and 5 mM DTT at 37°C for 2 hours.
2.2 Terminate the reaction by precipitating with acetone (1 mL of cold acetone, incubated at -80°C for 1 hour, then centrifuged at 20,000 g for 20 minutes).
2.3 Resuspend the precipitate in the SDS-PAGE sample buffer.
3. Detection: Analyzed using streptavidin blotting (Western Blot).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 1176905-54-6
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性状 Solid
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分子量 195.22
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分子式 C9H13N3O2
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Color White to off-white
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SMILES
[N-]=[N+]=NCCCC1C(CC(CC1)=O)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 50 mg/mL (256.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.1224 mL | 25.6121 mL | 51.2243 mL | 128.0606 mL |
| 5 mM | 1.0245 mL | 5.1224 mL | 10.2449 mL | 25.6121 mL | |
| 10 mM | 0.5122 mL | 2.5612 mL | 5.1224 mL | 12.8061 mL | |
| 15 mM | 0.3415 mL | 1.7075 mL | 3.4150 mL | 8.5374 mL | |
| 20 mM | 0.2561 mL | 1.2806 mL | 2.5612 mL | 6.4030 mL | |
| 25 mM | 0.2049 mL | 1.0245 mL | 2.0490 mL | 5.1224 mL | |
| 30 mM | 0.1707 mL | 0.8537 mL | 1.7075 mL | 4.2687 mL | |
| 40 mM | 0.1281 mL | 0.6403 mL | 1.2806 mL | 3.2015 mL | |
| 50 mM | 0.1024 mL | 0.5122 mL | 1.0245 mL | 2.5612 mL | |
| 60 mM | 0.0854 mL | 0.4269 mL | 0.8537 mL | 2.1343 mL | |
| 80 mM | 0.0640 mL | 0.3202 mL | 0.6403 mL | 1.6008 mL | |
| 100 mM | 0.0512 mL | 0.2561 mL | 0.5122 mL | 1.2806 mL |
DAz-2 関連分類
HY-132278 関連分類
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