DBCO-PEG8-NHS ester
Based on 1 Customer Validation
DBCO-PEG8-NHS ester is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs. DBCO-PEG8-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.34%
- CAS 番号: 2553412-88-5
- 分子式: C42H55N3O14
- 分子量:825.90
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保管条件:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
PROTAC Linkers アイソフォーム固有の製品をすべて表示
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生物活性
製品説明
IC50 & Target
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PEGs |
Alkyl/ether |
体外実験
PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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CAS 番号 2553412-88-5
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性状 Oil
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分子量 825.90
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分子式 C42H55N3O14
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Color Light yellow to yellow
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SMILES
O=C(N1CC2=C(C#CC3=C1C=CC=C3)C=CC=C2)CCC(NCCOCCOCCOCCOCCOCCOCCOCCOCCC(ON4C(CCC4=O)=O)=O)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
DMSO : 200 mg/mL (242.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
プロトコル
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
純度とドキュメンテーション
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データシート (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2108 mL | 6.0540 mL | 12.1080 mL | 30.2700 mL |
| 5 mM | 0.2422 mL | 1.2108 mL | 2.4216 mL | 6.0540 mL | |
| 10 mM | 0.1211 mL | 0.6054 mL | 1.2108 mL | 3.0270 mL | |
| 15 mM | 0.0807 mL | 0.4036 mL | 0.8072 mL | 2.0180 mL | |
| 20 mM | 0.0605 mL | 0.3027 mL | 0.6054 mL | 1.5135 mL | |
| 25 mM | 0.0484 mL | 0.2422 mL | 0.4843 mL | 1.2108 mL | |
| 30 mM | 0.0404 mL | 0.2018 mL | 0.4036 mL | 1.0090 mL | |
| 40 mM | 0.0303 mL | 0.1514 mL | 0.3027 mL | 0.7568 mL | |
| 50 mM | 0.0242 mL | 0.1211 mL | 0.2422 mL | 0.6054 mL | |
| 60 mM | 0.0202 mL | 0.1009 mL | 0.2018 mL | 0.5045 mL | |
| 80 mM | 0.0151 mL | 0.0757 mL | 0.1514 mL | 0.3784 mL | |
| 100 mM | 0.0121 mL | 0.0605 mL | 0.1211 mL | 0.3027 mL |
DBCO-PEG8-NHS ester 関連分類
HY-140273 関連分類
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