DBNBS
DBNBS is a radical scavenger. DBNBS undergoes non-radical nucleophilic addition to bind directly to cysteine (e.g., Cys-34 of BSA) to form a hydroxylamine, which is subsequently oxidized to produce an artifact spin adduct signal. DBNBS can be used for detecting biological free radicals.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 83016-63-1
- 分子式: C6H3Br2NO4S
- 分子量:344.97
-
保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
DBNBS (2.5-5 mM; overnight) forms an artificial artifact nitroxide adduct with the Cys-34 thiol residue of BSA via non-radical nucleophilic addition followed by oxidation, whereas its tertiary carbon-centered radical adduct formed with photochemically oxidized BSA arises from genuine spin trapping[1].
DBNBS (2.5 mM; 1 min-2 h) rapidly and completely depletes the free Cys-34 thiol content of bovine serum albumin, with nearly complete depletion observable within 1 minute of incubation[1].
DBNBS (3.7 μM; 1 min-2 h) quenches the tryptophan fluorescence in bovine serum albumin through binding rather than chemical reactions, and exerts no effect on the fluorescence of free tryptophan under the tested conditions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 83016-63-1
-
分子量 344.97
-
分子式 C6H3Br2NO4S
-
SMILES
O=NC1=C(C=C(S(=O)(O)=O)C=C1Br)Br
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
[1]. Silvester JA, et al. A study of photochemically-generated protein radical spin adducts on bovine serum albumin: the detection of genuine spin-trapping and artefactual, non-radical addition in the same molecule. Redox report : communications in free radical research. 1997 Aug;3(4):225-31. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
DBNBS 関連分類
HY-180497 関連分類
Powered by Bioz