Direct Red 80
Based on 2 publication(s) in Google Scholar
Direct Red 80 (Sirius Red) is a polyazo dye used principally in staining methods for collagen and amyloid. Direct Red 80 does not release benzidine upon degradation and is safer than many traditional direct dyes.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 95.0%
- CAS 番号: 2610-10-8
- 分子式: C45H26N10Na6O21S6
- 分子量:1373.07
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保管条件:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
MedChemExpress(MCE)の使用を引用している文献 Direct Red 80
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生物活性
Direct Red 80 (Sirius Red) has two important uses in histopathology: staining collagen from solution in saturated aqueous picric acid and staining amyloid from an alkaline solution. Stained structures are red[1].
Confocal laser microscopy (CLM) is also used to evaluate collagen production and distribution after Direct Red 80 staining. Direct Red 80 is not specific for the different types of collagen. Samples are washed in dH2O, pretreats for 40 min with a solution of 0.2% phosphomolybdic acid (PMA) and incubates for 30 min in a solution consisting of 0.1% Direct red 80 diluted in saturated aqueous picric acid. Finally each sample is washed in 0.01 M HCl for 2 min. PMA is used to make colourless cytoplasm and HCl is used to remove non-specific staining[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 2610-10-8
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性状 Solid
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分子量 1373.07
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分子式 C45H26N10Na6O21S6
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Color Brown to reddish brown
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SMILES
O=C(NC1=CC2=CC(S(=O)(O[Na])=O)=C(/N=N/C3=CC=C(/N=N/C4=CC=C(S(=O)(O[Na])=O)C=C4)C=C3S(=O)(O[Na])=O)C(O)=C2C=C1)NC5=CC6=CC(S(=O)(O[Na])=O)=C(/N=N/C7=CC=C(/N=N/C8=CC=C(S(=O)(O[Na])=O)C=C8)C=C7S(=O)(O[Na])=O)C(O)=C6C=C5
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別名
Sirius Red
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (2)
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Journal Impact Factor
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Most Recent
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J Extracell Vesicles
Umbilical Cord-Mesenchymal Stromal Cell-Derived Extracellular Vesicles Target the Liver to Improve Neurovascular Health in Type 2 Diabetes With Non-Alcoholic Fatty Liver Disease. [Abstract]2025 Jul;14(7):e70125. PMID: 40620065 -
溶剤 & 溶解度
DMSO : 5 mg/mL (3.64 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (271 KB)
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SDS (537 KB)
- English - EN (537 KB)
- Français - FR (537 KB)
- Deutsch - DE (537 KB)
- Norwegian - NO (537 KB)
- Español - ES (537 KB)
- Swedish - SV (537 KB)
- Italian - IT (537 KB)
- Portuguese - PT (537 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7283 mL | 3.6415 mL | 7.2830 mL | 18.2074 mL |