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United States

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製品番号: HY-189158

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DMA-132

DMA-132 is a translation inhibitor and antiviral agent. DMA-132 can target and bind to the conserved RNA stem-loop (bulge structure) in the 5'-UTR of SARS-CoV-2, thereby blocking viral RNA translation and replication. DMA-132 can be used in research related to coronavirus disease 2019 (COVID-19) and coronavirus infections.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 1160-14-1
分子式: C₁₄H₁₇N₇O
分子量:299.33

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

DMA-132 構造式

CAS 番号: 1160-14-1

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

Cellular Effect

Cell Line	Type	Value	Description	References
Vero E6	CC₅₀	>100 μM	Cytotoxicity against Vero E6 cells assessed as reduction in cell viability incubated for 96 hrs at 33°C by MTT assay. Cytotoxicity against Vero E6 cells assessed as reduction in cell viability incubated for 96 hrs at 33°C by MTT assay.	34826236

体外実験

DMA-132 (50-100 μM; 24 h) reduces the viral titer by approximately 1000-fold in Vero E6 cells infected with hCoV OC43^[1].
DMA-132 (1 μM-1 mM; 24-72 h) reduces infectious virus titers and decreases viral RNA levels in cell supernatants in a dose-dependent manner in SARS-CoV-2-infected Vero E6 cells^[1].
DMA-132 (0.1-100 μM; 48 h) reduces 5'-UTR-dependent luciferase expression in Vero E6 cells co-transfected with the SARS-CoV-2 5'-UTR-FLuc luciferase reporter gene^[1].
DMA-132 (500 μM) specifically binds to RNA structures (mainly the internal bulge regions of SL1, SL4, SL5a and SL6) in a cell-free system^[1].
DMA-132 (1 μM-1 mM; 96 h) exhibits extremely low cytotoxicity in Vero E6 cells, with a CC50 > 100 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

Vero E6 cells
Concentration:

0.01 nM, 0.1 nM, 1 nM, 0.01 μM, 0.1 μM, 1 μM, 10 μM, 100 μM, 1 mM
Incubation Time:

96 h
Result:

Did not substantially reduce cell viability at concentrations < 100 μM, revealing a 50% cytotoxic concentration (CC₅₀) of > 100 μM.

Real Time qPCR^[1]

Cell Line:

Vero E6 cells
Concentration:

1 μM, 10 μM, 100 μM, 1 mM
Incubation Time:

24, 48, 72 h
Result:

Dose-dependently decreased cell-free viral RNA levels in supernatants of infected cells.

化学情報

CAS 番号 1160-14-1
分子量 299.33
分子式 C₁₄H₁₇N₇O
SMILES

O=C(C1=C(N)N=C(N(C)C)C(C2=CC=CC=C2)=N1)NC(N)=N
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Zafferani M, et al. Amilorides inhibit SARS-CoV-2 replication in vitro by targeting RNA structures. Science advances. 2021 Nov 26;7(48):eabl6096.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

Minimum order quantity

100 mg

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