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United States

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製品番号: HY-N8933

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Glucolimnanthin

Glucolimnanthin is a plant glucoside that can be isolated from Limnanthes douglasii. Glucolimnanthin can increase metabolic activation of benzo[a]pyrene (B[a]P) in cultured mammalian cells

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CAS 番号: 111810-95-8
分子式: C₁₅H₂₁NO₁₀S₂
分子量:439.46

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

Glucolimnanthin 構造式

CAS 番号: 111810-95-8

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

Glucolimnanthin is a plant glucoside that can be isolated from Limnanthes douglasii. Glucolimnanthin can increase metabolic activation of benzo[a]pyrene (B[a]P) in cultured mammalian cells^[1]

化学情報

CAS 番号 111810-95-8
分子量 439.46
分子式 C₁₅H₂₁NO₁₀S₂
SMILES

COC1=CC=CC(C/C(S[C@@H]2O[C@@H]([C@H]([C@@H]([C@H]2O)O)O)CO)=N\OS(=O)(O)=O)=C1
Structure Classification
- Glucosinolates
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

プロトコル

All Molecular Biology

CRISPR-Cas9 knockout in cultured mammalian cells
CRISPR-Cas9 knockout in cultured mammalian cells uses an sgRNA to direct Cas9 to a complementary genomic sequence adjacent to a compatible PAM; Cas9 creates a targeted DNA double-strand break, and repair by non-homologous end joining can introduce insertions or deletions that disrupt the coding sequence or functional genomic element. The readout of knockout is detection of edited alleles and loss of gene product or phenotype, commonly by PCR/Sanger-sequence trace decomposition, targeted sequencing, immunoblotting, immunostaining, or flow cytometry when the target protein is detectable at the cell surface.
Detergent-Based Total Protein Extraction from Cultured Mammalian Cells (RIPA/NP-40/Triton Lysis)
Detergent-based total protein extraction lyses cultured mammalian cells by using amphipathic detergents to disrupt lipid membranes and solubilize proteins into aqueous lysate; RIPA combines non-ionic detergent with ionic detergents, whereas NP-40/Triton buffers are milder non-ionic lysis conditions. The readout is the recovered clarified protein lysate, which is commonly quantified by detergent-compatible protein assays and then used for downstream protein analysis such as SDS-PAGE and Western blotting.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (269 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Baird WM, et al. Glucolimnanthin, a plant glucosinolate, increases the metabolism and DNA binding of benzo[a]pyrene in hamster embryo cell cultures. Carcinogenesis. 1988 Apr;9(4):657-60. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Keywords

MOQ

Minimum order quantity

100 mg

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