GP515

GP515 potently and specifically inhibits isolated human cardiac adenosine kinase with an IC₅₀ of 4 nM^[2].
GP515 (2-20 μM; 18 h) increases VEGF mRNA expression in cultured rat myocardial myoblasts by 1.67-fold and 1.82-fold, respectively, after 18 h of normoxic incubation^[2].
GP515 (0.2-200 μM; 18 h) induces a dose-related increase in VEGF protein expression in cultured rat myocardial myoblasts after 18 h of normoxic incubation, reaching up to a 54% increase at the highest concentration^[2].
GP515 (1 μM; 12-24 h) significantly increases VEGF protein expression in cultured rat myocardial myoblasts after 12 h of normoxic incubation, with effects persisting through 24 h^[2].
GP515 (2 μM; 18 h) co-incubated with adenosine deaminase completely blocks the VEGF protein-inducing effect in cultured rat myocardial myoblasts after 18 h of normoxic incubation^[2].
GP515 (20 μM; 24 h) stimulates human umbilical vein endothelial cell proliferation by 98% and increases [³H]thymidine incorporation by 82% after 24 h of incubation, with no effect on rat myocardial myoblast proliferation^[2].
GP515 (20 μM; 18 h) increases VEGF protein expression in cultured rat myocardial myoblasts by 37% under normoxia and by an additional 27% under mild hypoxia (10% O₂) after 18 h of incubation, but has no effect under severe hypoxia (1% O₂)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.