GRGESP
Based on 1 Customer Validation
GRGESP is a collagen gel contraction inhibitor. GRGESP inhibits the spreading of human fibroblasts inside collagen gels and markedly decreased gel contraction. GRGDSP can be used for the research of connective tissue morphogenesis.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.82%
- CAS 番号: 99896-88-5
- 分子式: C23H39N9O10
- 分子量:601.61
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
生物活性
製品説明
体外実験
GRGESP inhibits the spreading of human fibroblasts inside collagen gels and markedly decreased gel contraction[1].
GRGDSP inhibits cell spreading on collagen-coated surfaces[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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CAS 番号 99896-88-5
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性状 Solid
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分子量 601.61
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分子式 C23H39N9O10
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Color White to off-white
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シーケンスの短縮
GRGESP
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
H2O : 50 mg/mL (83.11 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
プロトコル
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Connective Tissue: Masson's Trichrome/Collagen Trichrome Staining
Masson’s Trichrome (collagen/trichrome staining) is a histological technique that differentially stains tissue compartments using sequential acidic dyes to distinguish collagen from muscle and cytoplasmic components based on dye affinity and tissue permeability differences, enabling visualization of fibrosis and connective tissue architecture in histological sections. The classical formulation typically uses Weigert's iron hematoxylin for nuclear staining, Biebrich scarlet-acid fuchsin for cytoplasm and muscle, and aniline blue (or light green variants) for collagen, producing a characteristic blue/green collagen signal contrasted against red cytoplasm and dark nuclei. The staining principle relies on selective displacement of smaller dye molecules by larger anionic dyes in collagen-rich regions under controlled acidified conditions, which enhances collagen-specific dye retention. This property makes the method widely used for fibrosis assessment in organs such as heart, liver, lung, a
純度とドキュメンテーション
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データシート (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.6622 mL | 8.3110 mL | 16.6221 mL | 41.5552 mL |
| 5 mM | 0.3324 mL | 1.6622 mL | 3.3244 mL | 8.3110 mL | |
| 10 mM | 0.1662 mL | 0.8311 mL | 1.6622 mL | 4.1555 mL | |
| 15 mM | 0.1108 mL | 0.5541 mL | 1.1081 mL | 2.7703 mL | |
| 20 mM | 0.0831 mL | 0.4156 mL | 0.8311 mL | 2.0778 mL | |
| 25 mM | 0.0665 mL | 0.3324 mL | 0.6649 mL | 1.6622 mL | |
| 30 mM | 0.0554 mL | 0.2770 mL | 0.5541 mL | 1.3852 mL | |
| 40 mM | 0.0416 mL | 0.2078 mL | 0.4156 mL | 1.0389 mL | |
| 50 mM | 0.0332 mL | 0.1662 mL | 0.3324 mL | 0.8311 mL | |
| 60 mM | 0.0277 mL | 0.1385 mL | 0.2770 mL | 0.6926 mL | |
| 80 mM | 0.0208 mL | 0.1039 mL | 0.2078 mL | 0.5194 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.