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United States

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製品番号: HY-D0121

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INDO 1

INDO 1 is a cell-impermeable, dual-emission ratiometric fluorescent calcium indicator with a K_D·β value of 0.44 μM. INDO 1 binds to intracellular free calcium ions and undergoes wavelength-dependent fluorescence changes. In the unbound state (free form) without Ca²⁺, its fluorescence emission peak is at 485 nm upon excitation with ultraviolet light (350 nm). When INDO 1 pentasodium binds to Ca²⁺ (bound state), the emission peak shifts to 405 nm (Ex = 350 nm; dual emission at Em= 405/485 nm).

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CAS 番号: 96314-96-4
分子式: C₃₂H₃₁N₃O₁₂
分子量:649.60

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (274 KB)

INDO 1 構造式

CAS 番号: 96314-96-4

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- 体外実験
- Emission (Em)
- Excitation (Ex)
化学情報
純度とドキュメンテーション
参考文献
Calculators
Help & FAQs
関連分類
Bioz

生物活性

製品説明

体外実験

INDO 1 enables accurate measurement of absolute cytoplasmic Ca²⁺ levels in R1-R6 photoreceptors of white-eyed Oregon wild-type Drosophila melanogaster^[1].
INDO 1 binds extensively to proteins isolated from rabbit ventricular myocytes^[2].
INDO 1 is a calcium-sensitive fluorescent dye used in glass capillaries, with an apparent K_D·β value of 0.44 μM; as calcium concentration increases, its emission at 405 nm enhances while emission at 470 nm diminishes^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

475

Excitation (Ex)

350

化学情報

CAS 番号 96314-96-4
分子量 649.60
分子式 C₃₂H₃₁N₃O₁₂
SMILES

O=C(C1=CC2=C(C=C1)C=C(C3=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC4=CC(C)=CC=C4N(CC(O)=O)CC(O)=O)=C3)N2)O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (274 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Hardie RC, et al. INDO-1 measurements of absolute resting and light-induced Ca2+ concentration in Drosophila photoreceptors. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1996 May 01;16(9):2924-33.

[2]. Hove-Madsen L, et al. Indo-1 binding to protein in permeabilized ventricular myocytes alters its spectral and Ca binding properties. Biophysical journal. 1992 Jul;63(1):89-97.

[3]. Jacquemond V, et al. Indo-1 fluorescence signals elicited by membrane depolarization in enzymatically isolated mouse skeletal muscle fibers. Biophysical journal. 1997 Aug;73(2):920-8.

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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