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製品番号: HY-183454

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JFD00950

JFD00950 is a human FEN1 inhibitor with an IC₅₀ of 5.5 μM. JFD00950 binds directly to FEN1, alters the secondary structure of this protein, and competitively inhibits its flap cleavage activity by interfering with substrate binding. JFD00950 selectively reduces the viability of cancer cells. JFD00950 can be used in the research of colon cancer and breast cancer.

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CAS 番号: 882278-66-2
分子式: C₁₆H₆Cl₄O₃
分子量:388.03

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

JFD00950 構造式

CAS 番号: 882278-66-2

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

JFD00950 (1.25-50 μM; 20 min) potently inhibits purified human FEN1 flap cleavage activity with an IC₅₀ of 5.5 μM^[1].
JFD00950 directly binds to purified human FEN1 protein, as shown by significant quenching of the protein's intrinsic fluorescence at 5 μM^[1].
JFD00950 (5 μM; 20 min) acts as a competitive inhibitor of purified human FEN1, increasing the apparent K_m for the flap DNA substrate while leaving Vmax unchanged at 5 μM^[1].
JFD00950 (3.125-50 μM; 48 h) exhibits selective anti-proliferative activity against cancer cell lines, with the highest potency against DLD-1 colon cancer cells (IC₅₀ = 16.17 μM), and minimal activity against normal HEK-293 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

DLD-1, HT-29, HCT-116, MCF-7, MDAMB-231, HEK-293
Concentration:

3.125, 6.25, 12.5, 25, 50 μM
Incubation Time:

48 h
Result:

Inhibited cell growth by 66.60% in DLD-1 cells at 25 μM.
Inhibited cell growth by 50.26% in HT-29 cells at 25 μM.
Inhibited cell growth by 48.8% in HCT-116 cells at 25 μM.
Inhibited cell growth by 46.84% in MCF-7 cells at 25 μM.
Inhibited cell growth by 23.1% in MDAMB-231 cells at 25 μM.
Inhibited cell growth by 20.41% in HEK-293 cells at 25 μM.
Exhibited an IC₅₀ of 16.17 μM for DLD-1 cells.

化学情報

CAS 番号 882278-66-2
分子量 388.03
分子式 C₁₆H₆Cl₄O₃
SMILES

O=C1C(Cl)=C(C(C2=C1C=CC=C2)=O)OC3=CC(Cl)=C(C=C3Cl)Cl
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Deshmukh AL, et al. Identification of human flap endonuclease 1 (FEN1) inhibitors using a machine learning based consensus virtual screening. Molecular BioSystems. 2017;13(8):1630-1639.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Minimum order quantity

100 mg

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