K114
Based on 1 Customer Validation
K114, a fluorescent Congo Red analogue, binds tightly to amyloid fibrils with an EC50 of 20-30 nM. K114 is an efficient detector of semen-derived enhancer of virus infection (SEVI).
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.59%
- CAS 番号: 872201-12-2
- 分子式: C22H17BrO2
- 分子量:393.27
-
保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
-
HY-111263
NIAD-4
-
HY-103466
FM4-64
-
HY-DY1031
FM4-64 (solution)
-
HY-100200
SMER28
-
HY-D0940
H2DCFDA
-
HY-103465
FFN511
-
HY-D0025
7-Amino-4-methylcoumarin-3-acetic acid
-
HY-123051
Ac-WLA-AMC
-
HY-103465R
FFN511 (Standard)
-
HY-D1303
Marina blue
-
HY-103467
NPE-caged-HPTS sodium
-
HY-134620
Cyanine 5 NHS ester tetrafluoroborate
生物活性
K114's unusually low buffer fluorescence is due to self-quenching in sedimentable aggregates or micelles which upon interacting with amyloid fibrils undergo an enhancement in fluorescence intensity and shifts in the excitation and emission spectra[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
550
498
化学情報
-
CAS 番号 872201-12-2
-
性状 Solid
-
分子量 393.27
-
分子式 C22H17BrO2
-
Color Light yellow to yellow
-
SMILES
BrC1=CC(/C=C/C2=CC=C(O)C=C2)=CC=C1/C=C/C3=CC=C(O)C=C3
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
DMSO : 100 mg/mL (254.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
-
データシート (269 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. LeVine H 3rd, et al. Mechanism of A beta(1-40) fibril-induced fluorescence of (trans,trans)-1-bromo-2,5-bis(4-hydroxystyryl)benzene (K114). Biochemistry. 2005 Dec 6;44(48):15937-43. [Content Brief]
[2]. Selmani V, et al. K114 (trans, trans)-bromo-2,5-bis(4-hydroxystyryl)benzene is an efficient detector of cationic amyloid fibrils. Protein Sci. 2015 Mar;24(3):420-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5428 mL | 12.7139 mL | 25.4278 mL | 63.5696 mL |
| 5 mM | 0.5086 mL | 2.5428 mL | 5.0856 mL | 12.7139 mL | |
| 10 mM | 0.2543 mL | 1.2714 mL | 2.5428 mL | 6.3570 mL | |
| 15 mM | 0.1695 mL | 0.8476 mL | 1.6952 mL | 4.2380 mL | |
| 20 mM | 0.1271 mL | 0.6357 mL | 1.2714 mL | 3.1785 mL | |
| 25 mM | 0.1017 mL | 0.5086 mL | 1.0171 mL | 2.5428 mL | |
| 30 mM | 0.0848 mL | 0.4238 mL | 0.8476 mL | 2.1190 mL | |
| 40 mM | 0.0636 mL | 0.3178 mL | 0.6357 mL | 1.5892 mL | |
| 50 mM | 0.0509 mL | 0.2543 mL | 0.5086 mL | 1.2714 mL | |
| 60 mM | 0.0424 mL | 0.2119 mL | 0.4238 mL | 1.0595 mL | |
| 80 mM | 0.0318 mL | 0.1589 mL | 0.3178 mL | 0.7946 mL | |
| 100 mM | 0.0254 mL | 0.1271 mL | 0.2543 mL | 0.6357 mL |
K114 関連分類
HY-103470 関連分類
Powered by Bioz