MitoB bromide
Based on 1 Customer Validation
MitoB bromide is an exomarker of mitochondrial hydrogen peroxide. MitoB bromide is a mitochondria-targeted ratiometric probe, and can be used to measure levels of one major ROS, hydrogen peroxide, within living animals.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.8%
- CAS 番号: 1247025-84-8
- 分子式: C25H23BBrO2P
- 分子量:477.14
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保管条件:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
MitoB is rapidly taken up by mitochondria and converts to MitoP, which enables the quantification of H2O2[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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CAS 番号 1247025-84-8
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性状 Solid
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分子量 477.14
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分子式 C25H23BBrO2P
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Color White to off-white
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SMILES
OB(C1=CC=CC(C[P+](C2=CC=CC=C2)(C3=CC=CC=C3)C4=CC=CC=C4)=C1)O.[Br-]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
DMSO : 116.67 mg/mL (244.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
プロトコル
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
純度とドキュメンテーション
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データシート (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Salin K, et al. Using the MitoB method to assess levels of reactive oxygen species in ecological studies of oxidative stress. Sci Rep. 2017 Jan 24;7:41228. [Content Brief]
[2]. Cochemé HM, et al. Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila. Nat Protoc. 2012 Apr 19;7(5):946-58. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0958 mL | 10.4791 mL | 20.9582 mL | 52.3955 mL |
| 5 mM | 0.4192 mL | 2.0958 mL | 4.1916 mL | 10.4791 mL | |
| 10 mM | 0.2096 mL | 1.0479 mL | 2.0958 mL | 5.2396 mL | |
| 15 mM | 0.1397 mL | 0.6986 mL | 1.3972 mL | 3.4930 mL | |
| 20 mM | 0.1048 mL | 0.5240 mL | 1.0479 mL | 2.6198 mL | |
| 25 mM | 0.0838 mL | 0.4192 mL | 0.8383 mL | 2.0958 mL | |
| 30 mM | 0.0699 mL | 0.3493 mL | 0.6986 mL | 1.7465 mL | |
| 40 mM | 0.0524 mL | 0.2620 mL | 0.5240 mL | 1.3099 mL | |
| 50 mM | 0.0419 mL | 0.2096 mL | 0.4192 mL | 1.0479 mL | |
| 60 mM | 0.0349 mL | 0.1747 mL | 0.3493 mL | 0.8733 mL | |
| 80 mM | 0.0262 mL | 0.1310 mL | 0.2620 mL | 0.6549 mL | |
| 100 mM | 0.0210 mL | 0.1048 mL | 0.2096 mL | 0.5240 mL |
MitoB bromide 関連分類
HY-126474 関連分類
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