NIBR-LTSi
Based on 2 publication(s) in Google Scholar
NIBR-LTSi is an orally active and selective LATS kinase inhibitor. NIBR-LTSi can activate YAP signaling. NIBR-LTSi promotes stem cell proliferation, maintains stemness and blocks differentiation. NIBR-LTSi accelerates liver regeneration following extended hepatectomy in mice.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.97%
- 分子式: C18H20N4O
- 分子量:308.38
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 NIBR-LTSi
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Customer Validation & Images
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WB
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Cell Imaging/Staining
YAP アイソフォーム固有の製品をすべて表示
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生物活性
製品説明
IC50 & Target
LATS[1]
体外実験
NIBR-LTSi (0.1-10 μM; 2 h) inhibits YAP phosphorylation in JHH5 cells with an IC50 of 2.16 μM[1].
NIBR-LTSi (1-10 μM; 3-10 days) promotes keratinocyte proliferation, increases epidermal thickness and blocks keratinocyte differentiation in human 3D skin model[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Partial hepatectomy treated C57BL/6 WT mice[1]
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Dosage:30 and 100 mg/kg
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Administration:Oral administration (p.o.); single dose
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Result:Significantly induced hepatocyte proliferation in a dose-dependent manner.
化学情報
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性状 Solid
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分子量 308.38
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分子式 C18H20N4O
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Color White to off-white
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SMILES
C1(NC2=CC=C(C=C2)O[C@H]3CNCCC3)=CC=NC4=C1C=CN4
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell
2026 Jun 11;189(12):3541-3552.e18. PMID: 41999746 -
Biochim Biophys Acta Mol Cell Res
Senolytic elimination of senescent cells improved periodontal ligament stem cell-based bone regeneration partially through inhibiting YAP. [Abstract]2025 Feb 17;1872(3):119921. PMID: 39971252
NIBR-LTSi purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Cell Res. 2025 Feb 17;1872(3):119921. [Abstract]
NIBR-LTSi (2.5 μM). Western blot results of YAP and the senescence-related genes P21 and apoptosis-related genes BCL2 and BAX in PDLSCs.
NIBR-LTSi purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta Mol Cell Res. 2025 Feb 17;1872(3):119921. [Abstract]
NIBR-LTSi (2.5 μM). Microscopic observation and quantitative analysis of β-galactosidase-positive stained PDLSCs were performed. NC: P20 PDLSCs. +S: P20 PDLSCs were treated with senolytics. +S+N: P20 PDLSCs treated with senolytics plus NIBR-LTSi.
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (324.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (16.21 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (16.21 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (274 KB)
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SDS (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2428 mL | 16.2138 mL | 32.4275 mL | 81.0688 mL |
| 5 mM | 0.6486 mL | 3.2428 mL | 6.4855 mL | 16.2138 mL | |
| 10 mM | 0.3243 mL | 1.6214 mL | 3.2428 mL | 8.1069 mL | |
| 15 mM | 0.2162 mL | 1.0809 mL | 2.1618 mL | 5.4046 mL | |
| 20 mM | 0.1621 mL | 0.8107 mL | 1.6214 mL | 4.0534 mL | |
| 25 mM | 0.1297 mL | 0.6486 mL | 1.2971 mL | 3.2428 mL | |
| 30 mM | 0.1081 mL | 0.5405 mL | 1.0809 mL | 2.7023 mL | |
| 40 mM | 0.0811 mL | 0.4053 mL | 0.8107 mL | 2.0267 mL | |
| 50 mM | 0.0649 mL | 0.3243 mL | 0.6486 mL | 1.6214 mL | |
| 60 mM | 0.0540 mL | 0.2702 mL | 0.5405 mL | 1.3511 mL | |
| 80 mM | 0.0405 mL | 0.2027 mL | 0.4053 mL | 1.0134 mL | |
| 100 mM | 0.0324 mL | 0.1621 mL | 0.3243 mL | 0.8107 mL |
NIBR-LTSi 関連分類
HY-160769 関連分類
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