PFB-FDG
Based on 1 Customer Validation
PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=535 nm). PFB-FDG can be used for the determination of β-galactosidase activity.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.19%
- CAS 番号: 209540-64-7
- 分子式: C39H32F5NO16
- 分子量:865.66
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of β-galactosidase activity[1]:
1. Incubate 2 × 105 cells with 50 μg/mL of PFB-FDG in serum-free DMEM containing 25 mM Hepes (pH 7.4) for 2 h at 37°C. (Mononuclear cells for example, different cell concentrations vary)
2. Wash cells once with ice-cold PBS.
3. Analyze cells by flow cytometry (Ex = 485 nm, Em = 535 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
535
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化学情報
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CAS 番号 209540-64-7
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性状 Solid
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分子量 865.66
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分子式 C39H32F5NO16
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Color Light yellow to yellow
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SMILES
O[C@H]([C@H]1O)[C@@H](O)[C@@H](CO)O[C@H]1OC(C=C2)=CC3=C2C4(OC(C5=C4C=CC(NC(C6=C(F)C(F)=C(F)C(F)=C6F)=O)=C5)=O)C7=C(O3)C=C(O[C@@H]8O[C@H](CO)[C@H](O)[C@H](O)[C@H]8O)C=C7
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 100 mg/mL (115.52 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1552 mL | 5.7759 mL | 11.5519 mL | 28.8797 mL |
| 5 mM | 0.2310 mL | 1.1552 mL | 2.3104 mL | 5.7759 mL | |
| 10 mM | 0.1155 mL | 0.5776 mL | 1.1552 mL | 2.8880 mL | |
| 15 mM | 0.0770 mL | 0.3851 mL | 0.7701 mL | 1.9253 mL | |
| 20 mM | 0.0578 mL | 0.2888 mL | 0.5776 mL | 1.4440 mL | |
| 25 mM | 0.0462 mL | 0.2310 mL | 0.4621 mL | 1.1552 mL | |
| 30 mM | 0.0385 mL | 0.1925 mL | 0.3851 mL | 0.9627 mL | |
| 40 mM | 0.0289 mL | 0.1444 mL | 0.2888 mL | 0.7220 mL | |
| 50 mM | 0.0231 mL | 0.1155 mL | 0.2310 mL | 0.5776 mL | |
| 60 mM | 0.0193 mL | 0.0963 mL | 0.1925 mL | 0.4813 mL | |
| 80 mM | 0.0144 mL | 0.0722 mL | 0.1444 mL | 0.3610 mL | |
| 100 mM | 0.0116 mL | 0.0578 mL | 0.1155 mL | 0.2888 mL |
PFB-FDG 関連分類
HY-D1699 関連分類
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