Intake of Polydextrose Alters Hematology and the Profile of Short Chain Fatty Acids in Partially Gastrectomized Rats

  • Nutrients. 2018 Jun 20;10(6):792. doi: 10.3390/nu10060792.
Mariane Moreira Ramiro do Carmo  1 ,  Ulana Chaves Sarmento  2 ,  Leandro Fontoura Cavalheiro  3 ,  Anderson Fernandes  4 ,  Wander Fernando de Oliveira Filiú  5 ,  Karine de Cássia Freitas Gielow  6 ,  Deiler Sampaio Costa  7 ,  Adriana Conceiçon Guercio  8 ,  Valter Aragão do Nascimento  9 ,  Camila Fontoura Acosta Ribeiro  10 ,  Alinne Pereira de Castro  11 ,  Cristiano Marcelo Espinola Carvalho  12 ,  Daiana Novello  13 ,  Valfredo de Almeida Santos-Junior  14 ,  Priscila Neder Morato  15 ,  Jaime Amaya-Farfan  16 ,  Priscila Aiko Hiane  17 ,  Elisvânia Freitas Dos Santos  18
Affiliations
  • 1. Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil. [email protected].
  • 2. Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil. [email protected].
  • 3. Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil. [email protected].
  • 4. Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil. [email protected].
  • 5. Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil. [email protected].
  • 6. Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil. [email protected].
  • 7. Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil. [email protected].
  • 8. Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil. [email protected].
  • 9. Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil. [email protected].
  • 10. Universidade Católica Dom Bosco, Campo Grande, 79117-900 Mato Grosso do Sul, Brazil. [email protected].
  • 11. Universidade Católica Dom Bosco, Campo Grande, 79117-900 Mato Grosso do Sul, Brazil. [email protected].
  • 12. Universidade Católica Dom Bosco, Campo Grande, 79117-900 Mato Grosso do Sul, Brazil. [email protected].
  • 13. State University of Centro-Oeste, Guarapuava, 85040-080 Paraná, Brazil. [email protected].
  • 14. University of Campinas, 13083-862 Campinas, SP, Brazil. [email protected].
  • 15. State University of Mato Grosso do Sul, Dourados, 79804-970 Mato Grosso do Sul, Brazil. [email protected].
  • 16. University of Campinas, 13083-862 Campinas, SP, Brazil. [email protected].
  • 17. Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil. [email protected].
  • 18. Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil. [email protected].
PMID: 29925762 DOI: 10.3390/nu10060792
Abstract

Polydextrose (PDX) ingestion may increase the intestinal absorption of iron. This study evaluated the effects of 7.5% polydextrose supplementation on markers of iron uptake, transport and storage in partially gastrectomized rats. Half of a batch of 40 male Wistar rats (250 g) underwent Billroth II partial gastrectomy with anterior truncal vagotomy (GXT), while the Other half underwent sham gastrectomy (SHAM). At 7 postoperative days, the Animals were subdivided into four groups (n = 10): Sham Control and GXT Control (no polydextrose); Sham PDX and GXT PDX (with 7.5% PDX). The Animals were euthanized after 60 day of PDX treatment. Organ weight, cecal pH, the characterization and quantification of short-chain fatty acids (SCFA), hematological parameters, hepatic iron content and the expression of Ferroportin (FPT) in the jejunum, cecum, colon and liver were evaluated. PDX caused changes in the cecum of the supplemented Animals, where there was a decrease in pH, increase in cecal wall and marked production of SCFA, especially acetic and propionic acids (p < 0.05). Hepatic iron levels were lower in GXT Animals. PDX increased Hemoglobin (HGB) values by 29.2% and hematocrit (HCT) by 55.8% in the GXT PDX group compared to the GXT Control group. The GXT PDX group had lower hepatic FPT expression (p < 0.05). PDX led to increased SCFA concentration in the supplemented Animals. Considering that SCFAs play a central role in the increasing nutrients uptake, this mechanism may be involved in altering the hematology profile observed in these Animals but not enough to reverse iron deficiency anemia in post-gastrectomy rats.

Keywords
gastrectomy; iron-deficiency anemia; prebiotics; rats.
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