Pulchinenoside E2
Based on 1 Customer Validation
Pulchinenoside E2 is a triterpenoid saponin. Pulchinenoside E2 inhibits the phosphorylation of STAT3 and JAK2, blocks the nuclear translocation and transcriptional activity of STAT3, and suppresses STAT3-dependent mitochondrial oxidative phosphorylation. Pulchinenoside E2 inhibits invasion, migration and autophagy of triple-negative breast cancer cells. Pulchinenoside E2 can be used in research related to triple-negative breast cancer.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.28%
- CAS 番号: 244202-36-6
- 分子式: C53H86O21
- 分子量:1059.24
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保管条件:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
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JAK2 |
STAT3 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-375 | IC50 |
5.9 μM
Compound: 9
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Cytotoxicity against human A375 cells after 72 hrs by sulforhodamine B assay
Cytotoxicity against human A375 cells after 72 hrs by sulforhodamine B assay
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[PMID: 23639650] |
| A549 | IC50 |
7.9 μM
Compound: 9
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Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay
Cytotoxicity against human A549 cells after 72 hrs by sulforhodamine B assay
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[PMID: 23639650] |
| HL-60 | IC50 |
2.6 μg/mL
Compound: 8
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Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL-60 cells after 72 hrs by MTT assay
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[PMID: 10514313] |
| HL-60 | IC50 |
3.5 μM
Compound: 9
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Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
Cytotoxicity against human HL60 cells after 72 hrs by MTT assay
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[PMID: 23639650] |
Pulchinenoside E2 (0.75-1.5 μM; 24 h) induces lysosomal dysfunction in HS-578T and MDA-MB-231 TNBC cells, characterized by suppressed proteolytic activity, time-dependent degradation of mature CTSD, and lysosomal alkalinization[1].
Pulchinenoside E2 (0.75-1.5 μM; 24 h) dose-dependently increases GFP-LC3B puncta formation and impairs autophagic flux in HS-578T and MDA-MB-231 TNBC cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HS-578T, MDA-MB-231 TNBC cells
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Concentration:0.75, 1.5 μM
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Incubation Time:24 h
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Result:Dose-dependently increased the number of autophagic puncta and fluorescence intensity of GFP-LC3B in both cell lines.
Dose-dependently increased the LC3II/LC3I ratio and accumulation of the autophagy substrate p62.
| Species | Dose | Route | Tmax | Cmax | T1/2 | AUC0-t | MRTlast |
|---|---|---|---|---|---|---|---|
| Mice[1] | 5 mg/kg | i.p. | 6.30 h | 35.70 μg/mL | >12 h | 338.80 μg·h/mL | 6.67 h |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:M-NSG mice (female, 4-6 weeks old, tail vein injection of MDA-MB-231 cells to induce metastases)[2]
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Dosage:5 mg/kg; 10 mg/kg
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Administration:i.p.; alternate days; 30 days
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Result:Markedly reduced pulmonary and hepatic metastatic lesion numbers and tumor volumes in mice treated with 10 mg/kg compared to controls.
Significantly decreased phosphorylated STAT3 (Tyr705/Ser727) expression in metastatic tumors via immunohistochemical staining.
Accumulated LC3B and p62 in metastatic tumors, consistent with autophagic flux blockade.
Maintained serum AST, ALT, CRE, and BUN levels comparable to controls.
Showed no significant histopathological damage in heart and kidney tissues.
Maintained stable body weight throughout treatment, indicating no overt toxicity.
化学情報
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CAS 番号 244202-36-6
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性状 Solid
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分子量 1059.24
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分子式 C53H86O21
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Color White to off-white
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SMILES
CC1(C)[C@@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@H](O[C@@H]4O[C@H](CO)[C@@H](O[C@@H]5O[C@H](CO)[C@@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@H]4O)[C@@H](O)[C@H](C)O3)[C@@H](O)[C@@H](O)CO2)CC[C@@]6(C)[C@@]1([H])CC[C@]7(C)[C@]6([H])CC=C8[C@@]7(C)CC[C@]9(C(O)=O)[C@@]8([H])CC(C)(C)CC9
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
純度とドキュメンテーション
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データシート (283 KB)
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SDS (252 KB)
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- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Pulchinenoside E2 関連分類
HY-N8098 関連分類
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