REF1 peptide
Based on 1 Customer Validation
REF1 peptide is a PORK1 agonist with an EC50 of 0.028 nM in tomato. REF1 peptide binds to the extracellular domain of PORK1, triggers receptor autophosphorylation, and drives downstream MPK3/MPK6 activation, thereby initiating defense and regeneration signaling cascades. REF1 peptide induces callus formation, inhibits shoot regeneration upon continuous exposure, and enhances the regeneration and transformation efficiency of recalcitrant dicotyledonous and monocotyledonous crops.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.95%
- CAS 番号: 1198397-79-3
- 分子式: C101H169N39O32
- 分子量:2441.66
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
REF1 peptide (100 nM; 12 h) potently induces the expression of the PI-II defense gene in wild-type Solanum lycopersicum seedlings[1].
REF1 peptide (1 nM; 7-14 d) restores the callus formation and shoot regeneration defects of hypocotyl explants from Solanum lycopersicum prp-2, prp-3 and spr9 mutants[1].
REF1 peptide (100 nM; 30 min) induces the activation of MPK3/6 in Solanum lycopersicum seedlings in a PORK1 receptor-dependent manner[1].
REF1 peptide (2 μg/mL; 24 h) increases the p53-mediated transcriptional activation levels of p21, Bax and GADD45 promoters by approximately 2-fold in H1299 cells[2].
REF1 peptide (20 μg/10-cm plate; 12-72 h) increases p53-induced endogenous p21 mRNA levels and elevates cyclin G mRNA levels in H1299 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:H1299 tetracycline-inducible p53 cell lines
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Concentration:pHook2-REF1 peptide vector (20 μg/10-cm plate)
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Incubation Time:72 h (transfection); 12 h (tetracycline maintenance/removal)
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Result:Further increased p53-induced p21 mRNA by ~40%.
Enhanced p53-induced cyclin G mRNA increase (2-fold without REF1 peptide) by 50-100%.
Had no effect on p21 or cyclin G mRNA in the absence of p53.
化学情報
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CAS 番号 1198397-79-3
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性状 Solid
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分子量 2441.66
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分子式 C101H169N39O32
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Color White to off-white
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配列
Ala-Thr-Asp-Arg-Arg-Gly-Arg-Pro-Pro-Ser-Arg-Pro-Lys-Val-Gly-Ser-Gly-Pro-Pro-Pro-Gln-Asn-Asn
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シーケンスの短縮
ATDRRGRPPSRPKVGSGPPPQNN
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
H2O : ≥ 100 mg/mL (40.96 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (279 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.4096 mL | 2.0478 mL | 4.0956 mL | 10.2389 mL |
| 5 mM | 0.0819 mL | 0.4096 mL | 0.8191 mL | 2.0478 mL | |
| 10 mM | 0.0410 mL | 0.2048 mL | 0.4096 mL | 1.0239 mL | |
| 15 mM | 0.0273 mL | 0.1365 mL | 0.2730 mL | 0.6826 mL | |
| 20 mM | 0.0205 mL | 0.1024 mL | 0.2048 mL | 0.5119 mL | |
| 25 mM | 0.0164 mL | 0.0819 mL | 0.1638 mL | 0.4096 mL | |
| 30 mM | 0.0137 mL | 0.0683 mL | 0.1365 mL | 0.3413 mL | |
| 40 mM | 0.0102 mL | 0.0512 mL | 0.1024 mL | 0.2560 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
REF1 peptide 関連分類
HY-P11056 関連分類
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