サフラニンO
Based on 1 Customer Validation
Safranin (Safranin T) is an important and classical phenazinium dye. Safranin has been extensively used in the academic field as a spectroscopic probe and indicator. Safranin possesses a planar structure and cationic charge. Safranin can readily intercalate into biological macromolecules, including DNA and proteins. Safranin has antibacterial effects against gram-positive bacteria (S. aureus). Safranin can be used as a redox indicator in the determination of metal ion concentration.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 477-73-6
- 分子式: C20H19ClN4
- 分子量:350.85
-
保管条件:
RT, sealed storage, away from moisture and light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
Safranin (0.1% (w/v), 1 min) differentially stains the cell cycle of plant cells[2].
Safranin (up to 2 μM) inhibits Complex I-driven OXPHOS (in mouse brain homogenates) capacity[3].
Safranin (0.025%) shows antibacterial activity against gram-positive bacteria (S. aureus)[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 477-73-6
-
性状 Solid
-
分子量 350.85
-
分子式 C20H19ClN4
-
Color Purple to black
-
SMILES
CC1=CC2=NC3=C([N+](C4=CC=CC=C4)=C2C=C1N)C=C(N)C(C)=C3.[Cl-]
-
別名
Safranin; Safranine T
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
RT, sealed storage, away from moisture and light
In solvent -80°C 1 year -20°C 6 months
溶剤 & 溶解度
DMSO : 25 mg/mL (71.26 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
-
データシート (274 KB)
-
SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Wan H, et al. Structure characterization and optical properties investigation of the four main components of the classical phenazinium dye Safranin O. Analyst. 2019;144(24):7149-7156. [Content Brief]
[2]. Swain D, et al. Differential staining of the cell cycle of plant cells using safranin and indigo-picrocarmine. Stain Technol. 1990;65(4):197-204. [Content Brief]
[3]. Krumschnabel G, et al. Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol. 2014;542:163-81. [Content Brief]
[4]. Al-Khikani F, et al. The Antibacterial Action of Safranin and Gentian Violet. Rambam Maimonides Med J. 2022 Jul 31;13(3):e0018. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8502 mL | 14.2511 mL | 28.5022 mL | 71.2555 mL |
| 5 mM | 0.5700 mL | 2.8502 mL | 5.7004 mL | 14.2511 mL | |
| 10 mM | 0.2850 mL | 1.4251 mL | 2.8502 mL | 7.1256 mL | |
| 15 mM | 0.1900 mL | 0.9501 mL | 1.9001 mL | 4.7504 mL | |
| 20 mM | 0.1425 mL | 0.7126 mL | 1.4251 mL | 3.5628 mL | |
| 25 mM | 0.1140 mL | 0.5700 mL | 1.1401 mL | 2.8502 mL | |
| 30 mM | 0.0950 mL | 0.4750 mL | 0.9501 mL | 2.3752 mL | |
| 40 mM | 0.0713 mL | 0.3563 mL | 0.7126 mL | 1.7814 mL | |
| 50 mM | 0.0570 mL | 0.2850 mL | 0.5700 mL | 1.4251 mL | |
| 60 mM | 0.0475 mL | 0.2375 mL | 0.4750 mL | 1.1876 mL |
Safranin 関連分類
HY-D0215 関連分類
Powered by Bioz