Sodium-binding benzofuran isophthalate
SBFI is a cell-impermeant, fluorescent Na+ indicator dye. SBFI is excited at 340 nm and the fluorophore emission is collected at 450 nm. SBFI selective for Na+ over K+ with Kd values of 20 and 120 mM for these ions, respectively. .
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- CAS 番号: 124549-08-2
- 分子式: C44H42N2O15
- 分子量:838.81
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
生物活性
The SBFI dye for each sample was prepared by : [1]
1. dissolving the appropriate quantity of dye powder in 3 mL of dimethyl sulphoxide.
2. The five solutions were agitated for 5 minutes.
3. Each sample was then exposed to ultraviolet light with a Leitz Orthoplan microscope, and fluorescence levels recorded.
4. Ten readings in total were achieved for each solution, agitating between readings.
5. For each set of sample results the highest, lowest, and median values were plotted against dye concentration.
6. From these tests the optimum fluorescence values, with minimum variation of upper and lower limits, was established with 50 g of SBFI dye.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 124549-08-2
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分子量 838.81
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分子式 C44H42N2O15
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SMILES
O=C(C1=CC=C(C(C(O)=O)=C1)C2=CC3=CC(OC)=C(N4CCOCCOCCN(CCOCC4)C5=C(OC)C=C6C=C(C7=CC=C(C(O)=O)C=C7C(O)=O)OC6=C5)C=C3O2)O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)