Tryptone
Based on 1 Customer Validation
Tryptone is a peptide-rich bacterial culture medium component that can regulate bacterial cell surface proteins and biofilm-related genes. Tryptone promotes the expression and assembly of bacterial adhesion proteins (such as LapA and LapF) by providing peptide substances as structural factors, enhancing cell surface hydrophobicity and intercellular adhesion, thereby stabilizing the biofilm matrix and supporting the maturation and maintenance of bacterial biofilms. The peptide mixture contained in Tryptone can specifically regulate the transcription of bacterial adhesion-related genes (such as activating LapA and inhibiting LapF), affecting the synthesis and localization of biofilm structural proteins.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 73049-73-7
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保管条件:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Tryptone (10 g/L; 10 min; 37°C) significantly improved the capture efficiency of T4 phage conjugated magnetic particles for Escherichia coli, and this effect was temperature-dependent[1].
In the Pseudomonas biofilm formation experiment, Tryptone (10 g/L; 24 h) promoted the formation of mature biofilms of wild-type and Fis-overexpressing strains of Pseudomonas putida, increasing the amount of biofilm by 1.3-2.9 times compared with the M9 medium group without Tryptone, and was related to peptide-mediated cell surface protein regulation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Escherichia coli (ATCC 11303)
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Concentration:10 g/L (added to Luria-Bertani medium or phosphate buffer)
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Incubation Time:10 min at 37°C
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Result:Increased the capture efficiency of T4 bacteriophage-conjugated Fe3O4 magnetic particles, with viable bacterial counts in the supernatant decreasing by 70% compared to Tryptone-free conditions.
Bacterial capture was temperature-dependent, with no significant effect observed at 4°C. Flow cytometry and plate counting assays showed that Tryptone facilitated irreversible binding between T4 bacteriophage and E. coli surface receptors, enhancing magnetic particle-mediated isolation of bacteria.
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Cell Line:Pseudomonas putida strains PSm (wild type) and F15 (fis-overexpression)
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Concentration:10 g/L (supplemented to M9-0.2CAA medium)
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Incubation Time:24 h
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Result:Increased biofilm formation measured by crystal violet staining, with wild-type PSm showing a 1.3-fold increase and F15 strain exhibiting a 2.9-fold increase compared to Tryptone-free M9 medium.
qPCR analysis revealed upregulated expression of adhesion genes lapA and downregulated lapF in F15 cells, while Western blot confirmed enhanced Fis protein levels in Tryptone-supplemented cultures.
Biofilm matrix stability was assessed via protease K treatment, showing reduced dispersion in Tryptone-containing media, indicating polypeptide-mediated reinforcement of extracellular matrix structure.
化学情報
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CAS 番号 73049-73-7
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性状 Solid
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Color Off-white to light yellow
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SMILES
[Tryptone]
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別名
ペプトン
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
溶剤 & 溶解度
H2O : 100 mg/mL (Need ultrasonic)
純度とドキュメンテーション
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データシート (272 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Liana AE, et al. T4 bacteriophage conjugated magnetic particles for E. coli capturing: Influence of bacteriophage loading, temperature and tryptone. Colloids Surf B Biointerfaces. 2017 Mar 1;151:47-57. [Content Brief]
[2]. Puhm M, et al. Tryptone in Growth Media Enhances Pseudomonas putida Biofilm. Microorganisms. 2022 Mar 14;10(3):618. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Tryptone 関連分類
HY-W133898 関連分類
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