2. Anti-infection Cell Cycle/DNA Damage
  3. SARS-CoV DNA/RNA Synthesis
  4. K22

K22 is an anti-Coronaviral compound. K22 reduces double-stranded RNA. K22 displays antiviral activity beyond the Coronavirinae subfamily, namely against nidoviruses of the Torovirinae subfamily (EToV and WBV) and members of the Arteriviridae family (PRRSV, EAV). K22 displays antiviral activity against HCoV-229E.

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K22

K22 Chemical Structure

CAS No. : 2141978-86-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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K22 Related Antibodies

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Description

K22 is an anti-Coronaviral compound. K22 reduces double-stranded RNA. K22 displays antiviral activity beyond the Coronavirinae subfamily, namely against nidoviruses of the Torovirinae subfamily (EToV and WBV) and members of the Arteriviridae family (PRRSV, EAV). K22 displays antiviral activity against HCoV-229E[1][2].

In Vitro

K22 (0.1-100 μM; 3 h inoculation) potently inhibits HCoV-229E plaque formation in MRC-5 cells with an IC50 of 0.7 μM[1].
K22 (20 μM; 6 h) does not impact Rapamycin (HY-10219)-induced cellular autophagy in Huh7 cells at a concentration of 20 μM[1].
K22 (40 μM; 4 h) potently inhibits EToV replication in E-Derm cells, with a 3.5 log10 reduction in viral titre[2].
K22 (30-50 μM; 4 h) dose-dependently inhibits WBV replication in EPC cells, with a 1.6 log10 reduction in viral RNA at 50 μM[2].
K22 (10-40 μM; 4 h pre-infection, 48 h post-infection) potently inhibits PRRSV replication in MARC-145 cells, with a maximal 6.1 log10 reduction in viral titre[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

K22 Related Antibodies

Cell Viability Assay[1]

Cell Line: MRC-5 cells
Concentration: 0.032-500 μM
Incubation Time: 48 h
Result: Does not reduce MRC-5 cell viability by >50% at a concentration range of 0.032-500 µM. Reduced MRC-5 cell proliferation with a 50% cytotoxic concentration (CC50) of 110 μM, resulting in a selective index (CC50/IC50) of 157.
Molecular Weight

505.40

Formula

C27H25BrN2O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N/C(C(N1CCC(O)(C2=CC=C(Br)C=C2)CC1)=O)=C\C3=CC=CC=C3)C4=CC=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (98.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9786 mL 9.8932 mL 19.7863 mL
5 mM 0.3957 mL 1.9786 mL 3.9573 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9786 mL 9.8932 mL 19.7863 mL 49.4658 mL
5 mM 0.3957 mL 1.9786 mL 3.9573 mL 9.8932 mL
10 mM 0.1979 mL 0.9893 mL 1.9786 mL 4.9466 mL
15 mM 0.1319 mL 0.6595 mL 1.3191 mL 3.2977 mL
20 mM 0.0989 mL 0.4947 mL 0.9893 mL 2.4733 mL
25 mM 0.0791 mL 0.3957 mL 0.7915 mL 1.9786 mL
30 mM 0.0660 mL 0.3298 mL 0.6595 mL 1.6489 mL
40 mM 0.0495 mL 0.2473 mL 0.4947 mL 1.2366 mL
50 mM 0.0396 mL 0.1979 mL 0.3957 mL 0.9893 mL
60 mM 0.0330 mL 0.1649 mL 0.3298 mL 0.8244 mL
80 mM 0.0247 mL 0.1237 mL 0.2473 mL 0.6183 mL
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K22 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

K222141978-86-9K 22K-22SARS-CoVDNA/RNA SynthesisSARS coronavirusviral RNA synthesisMERS-CoVdouble membrane vesiclensp6PRRSVcoronavirus non-structural protein 6primary human epithelia culturesMRC-5 cellsHCoV-229Edouble-stranded viral RNA replication intermediatesInhibitorinhibitorinhibit

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