K22
Based on 1 Customer Validation
K22 is an anti-Coronaviral compound. K22 reduces double-stranded RNA. K22 displays antiviral activity beyond the Coronavirinae subfamily, namely against nidoviruses of the Torovirinae subfamily (EToV and WBV) and members of the Arteriviridae family (PRRSV, EAV). K22 displays antiviral activity against HCoV-229E.
For research use only. We do not sell to patients.
- Purity: 98.46%
- CAS No.: 2141978-86-9
- Formula: C27H25BrN2O3
- Molecular Weight:505.40
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
K22 (0.1-100 μM; 3 h inoculation) potently inhibits HCoV-229E plaque formation in MRC-5 cells with an IC50 of 0.7 μM[1].
K22 (20 μM; 6 h) does not impact Rapamycin (HY-10219)-induced cellular autophagy in Huh7 cells at a concentration of 20 μM[1].
K22 (40 μM; 4 h) potently inhibits EToV replication in E-Derm cells, with a 3.5 log10 reduction in viral titre[2].
K22 (30-50 μM; 4 h) dose-dependently inhibits WBV replication in EPC cells, with a 1.6 log10 reduction in viral RNA at 50 μM[2].
K22 (10-40 μM; 4 h pre-infection, 48 h post-infection) potently inhibits PRRSV replication in MARC-145 cells, with a maximal 6.1 log10 reduction in viral titre[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MRC-5 cells
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Concentration:0.032-500 μM
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Incubation Time:48 h
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Result:Does not reduce MRC-5 cell viability by >50% at a concentration range of 0.032-500 µM. Reduced MRC-5 cell proliferation with a 50% cytotoxic concentration (CC50) of 110 μM, resulting in a selective index (CC50/IC50) of 157.
Chemical Information
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CAS No. 2141978-86-9
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Appearance Solid
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Molecular Weight 505.40
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Formula C27H25BrN2O3
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Color White to off-white
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SMILES
O=C(N/C(C(N1CCC(O)(C2=CC=C(Br)C=C2)CC1)=O)=C\C3=CC=CC=C3)C4=CC=CC=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (98.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Lundin A, et al. Targeting membrane-bound viral RNA synthesis reveals potent inhibition of diverse coronaviruses including the middle East respiratory syndrome virus. PLoS Pathog. 2014;10(5):e1004166. Published 2014 May 29. [Content Brief]
[2]. Rappe JCF, et al. Antiviral activity of K22 against members of the order Nidovirales. Virus Res. 2018;246:28-34. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9786 mL | 9.8932 mL | 19.7863 mL | 49.4658 mL |
| 5 mM | 0.3957 mL | 1.9786 mL | 3.9573 mL | 9.8932 mL | |
| 10 mM | 0.1979 mL | 0.9893 mL | 1.9786 mL | 4.9466 mL | |
| 15 mM | 0.1319 mL | 0.6595 mL | 1.3191 mL | 3.2977 mL | |
| 20 mM | 0.0989 mL | 0.4947 mL | 0.9893 mL | 2.4733 mL | |
| 25 mM | 0.0791 mL | 0.3957 mL | 0.7915 mL | 1.9786 mL | |
| 30 mM | 0.0660 mL | 0.3298 mL | 0.6595 mL | 1.6489 mL | |
| 40 mM | 0.0495 mL | 0.2473 mL | 0.4947 mL | 1.2366 mL | |
| 50 mM | 0.0396 mL | 0.1979 mL | 0.3957 mL | 0.9893 mL | |
| 60 mM | 0.0330 mL | 0.1649 mL | 0.3298 mL | 0.8244 mL | |
| 80 mM | 0.0247 mL | 0.1237 mL | 0.2473 mL | 0.6183 mL |
K22 Related Classifications
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