SPQ
Based on 1 Customer Validation
SPQ is a fluorescent dye. SPQ can be used to measure chloride ion concentration transients and transport rates in vesicles, cells, and even intact epithelium.
For research use only. We do not sell to patients.
- Purity: 98.82%
- CAS No.: 83907-40-8
- Formula: C13H15NO4S
- Molecular Weight:281.33
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
SPQ (1 mM or 10 mM, 16 h in 4°C or 30 min 37°C) can accurately and real-time report changes in intracellular Cl- concentration in human erythrocyte ghosts[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 83907-40-8
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Appearance Solid
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Molecular Weight 281.33
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Formula C13H15NO4S
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Color White to gray
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SMILES
O=S(CCC[N+]1=C2C=CC(OC)=CC2=CC=C1)([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
H2O : 25 mg/mL (88.86 mM; Need ultrasonic)
DMSO : < 1 mg/mL (insoluble or slightly soluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sermet-Gaudelus I, et al. Normal function of the cystic fibrosis conductance regulator protein can be associated with homozygous (Delta)F508 mutation. Pediatr Res. 2002 Nov;52(5):628-35. [Content Brief]
[2]. Illsley NP, et al. Membrane chloride transport measured using a chloride-sensitive fluorescent probe. Biochemistry. 1987 Mar 10;26(5):1215-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.5545 mL | 17.7727 mL | 35.5454 mL | 88.8636 mL |
| 5 mM | 0.7109 mL | 3.5545 mL | 7.1091 mL | 17.7727 mL | |
| 10 mM | 0.3555 mL | 1.7773 mL | 3.5545 mL | 8.8864 mL | |
| 15 mM | 0.2370 mL | 1.1848 mL | 2.3697 mL | 5.9242 mL | |
| 20 mM | 0.1777 mL | 0.8886 mL | 1.7773 mL | 4.4432 mL | |
| 25 mM | 0.1422 mL | 0.7109 mL | 1.4218 mL | 3.5545 mL | |
| 30 mM | 0.1185 mL | 0.5924 mL | 1.1848 mL | 2.9621 mL | |
| 40 mM | 0.0889 mL | 0.4443 mL | 0.8886 mL | 2.2216 mL | |
| 50 mM | 0.0711 mL | 0.3555 mL | 0.7109 mL | 1.7773 mL | |
| 60 mM | 0.0592 mL | 0.2962 mL | 0.5924 mL | 1.4811 mL | |
| 80 mM | 0.0444 mL | 0.2222 mL | 0.4443 mL | 1.1108 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
SPQ Related Classifications
HY-D0936 Related Classifications
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