3-WP

3-WP (32 μM; 24 h) acts synergistically with Melittin to enhance the antibacterial activity against multiple Gram-positive strains including MRSA ATCC43300, MRSA DL5F2 and MRSA DN65, with FICI values ranging from 0.125 to 0.250. 3-WP significantly reduces the geometric mean MIC of melittin from 2.333 μM to 0.250 μM, indicating that 3-WP effectively enhances the antibacterial efficacy of melittin^[1].
3-WP (1-64 μM; 4 h) exhibits favorable biosafety even at concentrations up to 64 μM, with no hemolysis or cytotoxicity; when used in combination with Melittin (HY-P0233), its cell selectivity is enhanced, and the SI against Gram-positive bacteria relative to mammalian cells increases to 53.827^[1].
Combination treatment with 3-WP (1 μM; 24 h) and 0.125 μM Melittin exerts synergistic bactericidal activity against MRSA ATCC43300, reducing bacterial counts by nearly 5-log₁₀ within 24 hours^[1].
Combination of 3-WP (passaged for 30 generations) and Melittin delays the development of bacterial resistance in MRSA ATCC43300, with its MIC increasing only 2-fold after 30 consecutive passages^[1].
Combination treatment with 3-WP (2 μM; 24 h) and 0.25 μM Melittin significantly inhibits biofilm formation of MRSA ATCC43300, reduces biofilm biomass and disrupts biofilm structure, whereas 3-WP used alone shows no such effects^[1].
3-WP (2-8 μM) binds to LTA and disrupts the cell wall permeability of MRSA ATCC43300 in a dose-dependent manner, and this activity is retained when used in combination with 0.25 μM Melittin^[1].
Combination treatment with 3-WP (2 μM; 0-120 min) and 0.25 μM Melittin significantly enhances cytoplasmic membrane damage to MRSA ATCC43300, increasing membrane rigidity, permeability, depolarization level and ATP leakage^[1].
3-WP (2-8 μM; 0.5-4 h) induces dose-dependent accumulation of ROS in MRSA ATCC43300 and inhibits its energy metabolism (intracellular ATP production and respiratory chain dehydrogenase activity)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.