4-Methylherniarin
Based on 1 Customer Validation
4-Methylherniarin (7-Methoxy-4-methylcoumarin) is a coumarin derivative and fluorescent label, has an antimicrobial activitiy against both gram positive and gram negative bacterial stains. 4-Methylherniarin displays good activity against B. subtilis and S.sonnei with IC50 values of 11.76 μg/ml and 13.47 μg/ml.
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- Purity: 99.71%
- CAS No.: 2555-28-4
- 화학식: C11H10O3
- 분자량:190.20
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보관:
4°C, protect from light.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
IC50 & Target
IC50: 11.76 μg/ml (B. subtilis); 13.47 μg/ml (S.sonnei)[1]
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| U-937 | CC50 |
>2000 μM
Compound: 11
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Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs
Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs
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[PMID: 18060791] |
| U-937 | IC50 |
>2000 μM
Compound: 11
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Growth inhibition of human U937 cells by [3H]thymidine incorporation assay
Growth inhibition of human U937 cells by [3H]thymidine incorporation assay
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[PMID: 18060791] |
Chemical Information
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CAS No. 2555-28-4
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Appearance Solid
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분자량 190.20
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화학식 C11H10O3
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Color White to off-white
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SMILES
O=C1C=C(C)C2=CC=C(OC)C=C2O1
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Synonyms
7-Methoxy-4-methylcoumarin
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, protect from light
In solvent -80°C 2 years -20°C 1 year
용액&용해도
In Vitro:
DMSO : 25 mg/mL (131.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.94 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (270 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.2576 mL | 26.2881 mL | 52.5762 mL | 131.4406 mL |
| 5 mM | 1.0515 mL | 5.2576 mL | 10.5152 mL | 26.2881 mL | |
| 10 mM | 0.5258 mL | 2.6288 mL | 5.2576 mL | 13.1441 mL | |
| 15 mM | 0.3505 mL | 1.7525 mL | 3.5051 mL | 8.7627 mL | |
| 20 mM | 0.2629 mL | 1.3144 mL | 2.6288 mL | 6.5720 mL | |
| 25 mM | 0.2103 mL | 1.0515 mL | 2.1030 mL | 5.2576 mL | |
| 30 mM | 0.1753 mL | 0.8763 mL | 1.7525 mL | 4.3814 mL | |
| 40 mM | 0.1314 mL | 0.6572 mL | 1.3144 mL | 3.2860 mL | |
| 50 mM | 0.1052 mL | 0.5258 mL | 1.0515 mL | 2.6288 mL | |
| 60 mM | 0.0876 mL | 0.4381 mL | 0.8763 mL | 2.1907 mL | |
| 80 mM | 0.0657 mL | 0.3286 mL | 0.6572 mL | 1.6430 mL | |
| 100 mM | 0.0526 mL | 0.2629 mL | 0.5258 mL | 1.3144 mL |
4-Methylherniarin Related Classifications
HY-D0128 Related Classifications
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