CHAPSO
Based on 1 Customer Validation
CHAPSO is a bile derivative. CHAPSO reduces protein adsorption, improves the cryo-EM imaging quality. CHAPSO can be used as a detergent, or as the membrane protein solubilization and reconstruction reagent in membrane protein structural studies.
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- Purity: 99.82%
- CAS No.: 82473-24-3
- 화학식: C32H58N2O8S
- 분자량:630.88
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보관:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
CHAPSO prevents bacterial transcription complexes from adsorbing on the air/water interface during cryo-EM grid preparation, thereby avoiding the generation of orientation bias. CHAPSO can be used for Cryo-EM sample preparation and Cryo-EM tomography[2].
CHAPSO forms a monolayer covering the air/water interface at the critical micelle concentration (CMC), changing the surface properties of the interface and preventing particle adsorption[2].
CHAPSO dissolves liposomes and membrane proteins, releasing them from lipid bilayer structures[3].
CHAPSO can be used to prepare phospholipid bilayers with specific orientations for nuclear magnetic resonance experiments[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 82473-24-3
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Appearance Solid
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분자량 630.88
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화학식 C32H58N2O8S
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Color White to off-white
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SMILES
O[C@H]1[C@@]2([H])[C@@]3([H])[C@@]([C@@](CC3)([H])[C@H](C)CCC(NCCC[N+](C)(C)CC(O)CS(=O)([O-])=O)=O)([C@H](C[C@]2([H])[C@@]4([C@](C[C@@H](CC4)O)([H])C1)C)O)C
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Synonyms
3-[3-(Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
용액&용해도
DMSO : 100 mg/mL (158.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (158.51 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Li M, et al. Morphological characterization of DMPC/CHAPSO bicellar mixtures: a combined SANS and NMR study. Langmuir. 2013 Dec 23;29(51):15943-57. [Content Brief]
[2]. Chen J, et al. Eliminating effects of particle adsorption to the air/water interface in single-particle cryo-electron microscopy: Bacterial RNA polymerase and CHAPSO. J Struct Biol X. 2019 Jan-Mar;1:100005. [Content Brief]
[3]. Cladera J, et al., Liposome solubilization and membrane protein reconstitution using Chaps and Chapso. Eur J Biochem. 1997 Feb 1;243(3):798-804. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.5851 mL | 7.9254 mL | 15.8509 mL | 39.6272 mL |
| 5 mM | 0.3170 mL | 1.5851 mL | 3.1702 mL | 7.9254 mL | |
| 10 mM | 0.1585 mL | 0.7925 mL | 1.5851 mL | 3.9627 mL | |
| 15 mM | 0.1057 mL | 0.5284 mL | 1.0567 mL | 2.6418 mL | |
| 20 mM | 0.0793 mL | 0.3963 mL | 0.7925 mL | 1.9814 mL | |
| 25 mM | 0.0634 mL | 0.3170 mL | 0.6340 mL | 1.5851 mL | |
| 30 mM | 0.0528 mL | 0.2642 mL | 0.5284 mL | 1.3209 mL | |
| 40 mM | 0.0396 mL | 0.1981 mL | 0.3963 mL | 0.9907 mL | |
| 50 mM | 0.0317 mL | 0.1585 mL | 0.3170 mL | 0.7925 mL | |
| 60 mM | 0.0264 mL | 0.1321 mL | 0.2642 mL | 0.6605 mL | |
| 80 mM | 0.0198 mL | 0.0991 mL | 0.1981 mL | 0.4953 mL | |
| 100 mM | 0.0159 mL | 0.0793 mL | 0.1585 mL | 0.3963 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
CHAPSO Related Classifications
HY-W040201 Related Classifications
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