EBV lytic cycle inducer-1
Based on 1 Customer Validation
Epstein-Barr virus (EBV) lytic cycle inducer-1 Dp44mT (compound C7) is an iron-chelatoe-like compound. Dp44mT cooperates with HDAC inhibitor Romidespin (HY-15149) and SAHA to induce EBV lytic cycle. Dp44mT reactivates EBV lytic cycle by activating the ERK1/2-autophagy axis in epithelial cancers.
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- Purity: 96.24%
- CAS No.: 394668-43-0
- 화학식: C14H12BrN3O
- 분자량:318.17
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
Dp44mT (compound C7) (0-80 μM; 48 h) induces lytic cycle in cell line-dependent manner, with higher toxicity in AGS-BX1 than in AGS[1].
Dp44mT (10 μM; 0-72 h) induces lytic cycle in a time-dependent manner[1].
Dp44mT (1.25-2.5 μM; 24 h) cooperates with HDAC inhibitor Romidespin and SAHA to induce EBV lytic cycle[1].
Dp44mT (20 μM; 48 h) leads to the EBV lytic cycle through induction of the ERK-autophagy axis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:AGS AGS-BX1
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Concentration:10 μM
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Incubation Time:24 h, 48 h, 72 h
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Result:Resulted the expression of IE proteins Zta, Rta, and early EBV lytic protein BMRF1 peaking at 24h post treatment.
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Cell Line:AGS-BX1
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Concentration:1.25 μM, 2.5 μM
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Incubation Time:24 h
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Result:Synergistically induced the expression of the viral IE protein Zta could together with 2.5 μM of SAHA and 2.5 nM of Rmidepsin.
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Cell Line:HA cells
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Concentration:20 μM
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Incubation Time:24 h
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Result:A significantly lower expression of Zta was observed in cells treated with the iron-precomplexed C7 when compared to cells treated with C7 with 41% higher.
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Cell Line:AGS, AGS-BX1
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Concentration:0 μM, 1.25 μM, 2.5 μM, 5 μM, 10 μM, 20 μM, 40 μM, 80 μM
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Incubation Time:48 h
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Result:Displayed significantly higher toxicity to the EBV-positive cell line AGS-BX1 than the EBV-negative counterpart.
Chemical Information
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CAS No. 394668-43-0
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Appearance Solid
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분자량 318.17
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화학식 C14H12BrN3O
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Color White to off-white
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SMILES
O=C(C1=CC(Br)=CC=C1)N/N=C(C)/C2=NC=CC=C2
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
DMSO : 12.5 mg/mL (39.29 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.56 mg/mL (1.76 mM); Clear solution
This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.56 mg/mL (1.76 mM); Clear solution
This protocol yields a clear solution of ≥ 0.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (275 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.1430 mL | 15.7149 mL | 31.4297 mL | 78.5743 mL |
| 5 mM | 0.6286 mL | 3.1430 mL | 6.2859 mL | 15.7149 mL | |
| 10 mM | 0.3143 mL | 1.5715 mL | 3.1430 mL | 7.8574 mL | |
| 15 mM | 0.2095 mL | 1.0477 mL | 2.0953 mL | 5.2383 mL | |
| 20 mM | 0.1571 mL | 0.7857 mL | 1.5715 mL | 3.9287 mL | |
| 25 mM | 0.1257 mL | 0.6286 mL | 1.2572 mL | 3.1430 mL | |
| 30 mM | 0.1048 mL | 0.5238 mL | 1.0477 mL | 2.6191 mL |
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