FlAsH-EDT2
Based on 2 publication(s) in Google Scholar
FlAsH-EDT2 is a protein labeling reagent. FlAsH-EDT2 binds to Cys4 with high affinity and emits fluorescence. FlAsH-EDT2 can cross the cell membrane and enter the interior of the cell. FlAsH-EDT2 can only be used for labeling proteins with high concentration.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 98.98%
- CAS No.: 212118-77-9
- 화학식: C24H18As2O5S4
- 분자량:664.50
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보관:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) FlAsH-EDT2
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Biological Activity
FlAsH-EDT2 binds to tetracysteine motif (CCXXCC, also called "Cys4"), causes the structure change of FlAsH-EDT2, allowing the absorption of light with specific wavelength and the fluorescence emission[1].
FlAsH-EDT2 also binds to non-specific cysteine-rich intracellular proteins, resulting in background fluorescence as false positive results. FlAsH-EDT2 is suggested to label recombinant proteins with high expression levels[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 212118-77-9
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Appearance Solid
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분자량 664.50
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화학식 C24H18As2O5S4
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Color White to light yellow
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SMILES
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4[As]5SCCS5)C([As]6SCCS6)=C(O)C=C3)C7=C1C=CC=C7
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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Mol Cell
2024 Feb 1;84(3):570-583.e7. PMID: 38215752 -
bioRxiv
Membrane binding promotes oligomerization and functional activation of viral RNA-dependent RNA polymerase. [Abstract]2025 Sep 8:2025.09.08.674988. PMID: 40964319
용액&용해도
DMSO : 25 mg/mL (37.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Stroffekova K, et al. The protein-labeling reagent FLASH-EDT2 binds not only to CCXXCC motifs but also non-specifically to endogenous cysteine-rich proteins. Pflugers Arch. 2001 Sep;442(6):859-66. [Content Brief]
[2]. Adams SR, et al. Preparation of the membrane-permeant biarsenicals FlAsH-EDT2 and ReAsH-EDT2 for fluorescent labeling of tetracysteine-tagged proteins. Nat Protoc. 2008;3(9):1527-34. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5049 mL | 7.5245 mL | 15.0489 mL | 37.6223 mL |
| 5 mM | 0.3010 mL | 1.5049 mL | 3.0098 mL | 7.5245 mL | |
| 10 mM | 0.1505 mL | 0.7524 mL | 1.5049 mL | 3.7622 mL | |
| 15 mM | 0.1003 mL | 0.5016 mL | 1.0033 mL | 2.5082 mL | |
| 20 mM | 0.0752 mL | 0.3762 mL | 0.7524 mL | 1.8811 mL | |
| 25 mM | 0.0602 mL | 0.3010 mL | 0.6020 mL | 1.5049 mL | |
| 30 mM | 0.0502 mL | 0.2508 mL | 0.5016 mL | 1.2541 mL |
FlAsH-EDT2 Related Classifications
HY-130412 Related Classifications
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