JMX0312
JMX0312 is a small-molecule salicylamide derivative with submicromolar anti-human adenovirus activity, with an IC50 of 0.18 μM. JMX0312 reduces viral loads in the liver and blood and decreases mortality in immunosuppressed hamsters infected with human adenovirus, and it can be used for research on human adenovirus infection.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- CAS No.: 439144-75-9
- 화학식: C14H8ClF4NO2
- 분자량:333.67
-
보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocol
- 순도&문서
- References
-
Help & FAQs
Biological Activity
제품 설명
IC50 & Target
[1]|
HAdV-5 0.18 μM (IC50) |
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A549 | CC50 |
120.0 μM
|
Cytotoxicity against A549 cells measured via AlamarBlue assay with 48-hour incubation.
Cytotoxicity against A549 cells measured via AlamarBlue assay with 48-hour incubation.
|
32045239 |
| A549 | IC50 |
2.23 μM
|
Inhibition of HAdV-5 entry in A549 cells assessed via GFP expression quantification with 48-hour incubation.
Inhibition of HAdV-5 entry in A549 cells assessed via GFP expression quantification with 48-hour incubation.
|
32045239 |
| A549 | IC50 |
0.18 μM
|
Inhibition of human adenovirus 5 (HAdV-5) replication in A549 cells measured by plaque reduction assay.
Inhibition of human adenovirus 5 (HAdV-5) replication in A549 cells measured by plaque reduction assay.
|
40185285 |
In Vitro
JMX0312 (50 μM; 48 h) completely inhibits the entry of HAdV-5 into A549 cells at a concentration of 50 μM. The entry inhibition IC50 in A549 cells is 2.23 μM, and the CC50 is 120.0 μM[1].
JMX0312 (50 μM) reduces the HAdV-5 viral titer in A549 cells by 989-fold[1].
JMX0312 (50 μM; 45 min) does not inhibit the delivery of the HAdV-5 genome to the nucleus under the treatment condition of administration at a concentration of 50 μM with continuous action for 45 min during A549 cell infection[1].
JMX0312 (50 μM; 24 h) does not significantly inhibit HAdV-5 DNA replication in A549 cells when administered at 25 μM for 24 h post-infection[1].
JMX0312 potently inhibits the replication of HAdV-5 in A549 cells, with an IC50 of 0.18 μM, a CC50 of 120.0 μM, and a selectivity index of 666.7[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:A549 cells
-
Concentration:50 μM
-
Incubation Time:45 min
-
Result:Showed no significant difference in the amount of nuclear-associated HAdV genomes compared to the DMSO control.
-
Cell Line:A549 cells
-
Concentration:50 μM
-
Incubation Time:24 h
-
Result:Showed low inhibition of HAdV-5 DNA replication compared to the DMSO control.
Parmacokinetics
| Species | Dose | Route | Cmax | T1/2 | AUC0-24 |
|---|---|---|---|---|---|
| Golden hamster[2] | 6.25 mg/kg | i.p. | 0.09 mg/L | 115.47 min | 919.05 mg/min/L |
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:RjHan/AURA (male, 7 weeks old, ~100 g, immunosuppressed with cyclophosphamide, inoculated with HAdV-C6)[2]
-
Dosage:6.25 mg/kg
-
Administration:i.p.; daily; 14 days
-
Result:Did not affect animal body weight.
Reduced liver viral load by 3.55 log10 PFU/g to a mean of 7.44 log10 PFU/g in infected animals (dead or euthanized over 14 days).
Reduced blood viral load by 7.49 log10 PFU/mL to a mean of 6.56 log10 PFU/mL in infected animals (dead or euthanized over 14 days).
Achieved a 14-day survival rate of 50%.
Reduced liver viral load to the assay detection limit of 3.5 log10 PFU/g in animals that survived 14 days.
Reduced blood viral load by 3.44 log10 PFU/mL to a mean of 2.67 log10 PFU/mL, just above the assay detection limit of 2.5 log10 PFU/mL, in animals that survived 14 days.
Chemical Information
-
CAS No. 439144-75-9
-
분자량 333.67
-
화학식 C14H8ClF4NO2
-
SMILES
O=C(C1=CC(Cl)=CC=C1O)NC2=CC(F)=CC(C(F)(F)F)=C2
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
JMX0312 Related Classifications
HY-187662 Related Classifications
Powered by Bioz